高等学校化学学报 ›› 1989, Vol. 10 ›› Issue (3): 308.

• 研究简报 • 上一篇    下一篇

刀豆组织电极用于脲测定的研究

沈国励1, 马铭1, 曾鸽鸣1, 俞汝勤1, 谢锦云2, 张梅芬2   

  1. 1. 湖南大学化学化工系;
    2. 湖南师范大学生物系
  • 收稿日期:1987-07-16 出版日期:1989-03-24 发布日期:1989-03-24
  • 通讯作者: 沈国励
  • 基金资助:

    国家自然科学基金

A Jack Bean Tissue Electrode for Urea Determination

Shen Guoli1, Ma Ming1, Zeng Geming1, Yu Ruqin1, Xie Jinyun2, Zhang Meifen2   

  1. 1. Department of Chemistry and Chemical Engineering, HunanUniversity, Chang sha;
    2. Department of Biology, Hunan Normal University, Changsha
  • Received:1987-07-16 Online:1989-03-24 Published:1989-03-24

摘要: 关于生物组织传感器的研究,Arnold有详细综述。刀豆中包含丰富的脲酶,本文报道将刀豆切片与自制石墨内导PVC膜pH电极型氨气敏电极偶合制成了刀豆切片脲电极,对其响应特性、介质条件、固定化及切片厚度等因素进行了研究,取得了较满意的结果。

关键词: 生物传感器, 组织切片电极, 脲的测定, 刀豆切片电极

Abstract: A jack bean tissue section electrode sensitive to urea has been prepared by coupling a jack bean slice with an ammonia gas-sensing probe using PVC membrane pHelectrode as the internal element. The potentiometric response characteristics of this new electrode under steady-state and dynamic condition were examined. The steady-state linear response ranse of this electrode is 1.0×10-2~4.6 ×10-5mol/L, with a detection limit of 4.0×10-5mol/L. The influence of some experimental parameters including buffer composition, operation pH, etc. are discussed. Urea was determined by using dynamic method, with an average recovery of 98.9% and a standard deviation of 1.8%.

Key words: Biosensor, Tissue section electrode, Determination of urea, Jack bean section electrode

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