高等学校化学学报 ›› 2013, Vol. 34 ›› Issue (5): 1072-1077.doi: 10.7503/cjcu20130075

• 分析化学 • 上一篇    下一篇

以对苯二甲酸为手臂连接剂的大肠杆菌DNA传感器

王立衡1, 高凤1, 蒋淑恋2, 丁应涛1, 郑德论1, 黄立漳2, 汪庆祥1   

  1. 1. 漳州师范学院化学与环境科学系, 漳州 363000;
    2. 漳州市产品质量监督检验所, 漳州 363000
  • 收稿日期:2013-01-22 出版日期:2013-05-10 发布日期:2013-05-10
  • 通讯作者: 汪庆祥, 男, 博士, 副教授, 主要从事生物电化学和化学生物传感器研究. E-mail: axiang236@126.com E-mail:axiang236@126.com
  • 基金资助:

    国家自然科学基金(批准号: 21275127)、国家质检总局科技计划项目(批准号: 2011QK217)、福建省教育厅重点项目(批准号: JA11166)和福建省高等学校新世纪优秀人才支持计划(批准号: JA12204)资助.

Escherichia Coli DNA Biosensor Using Terephthalic Acid as Arm Linker

WANG Li-Heng1, GAO Feng1, JIANG Shu-Lian2, DING Ying-Tao1, ZHENG De-Lun1, HUANG Li-Zhang2, WANG Qing-Xiang1   

  1. 1. Department of Chemistry and Environment Science, Zhangzhou Normal University, Zhangzhou 363000, China;
    2. Zhangzhou Product Quality Supervision and Inspection Institute, Zhangzhou 363000, China
  • Received:2013-01-22 Online:2013-05-10 Published:2013-05-10

摘要:

将空心球状CdS超声分散于聚乙烯醇(PVA)溶液中, 得到均匀的CdS-PVA复合材料分散液. 取适量分散液滴涂于玻碳电极表面, 晾干得到CdS-PVA修饰电极. 以对苯二甲酸为手臂连接剂, 在CdS-PVA膜上共价固定大肠杆菌特定寡聚核苷酸序列, 构建了一种新型的DNA传感器. 采用电化学阻抗法考察了该传感器的分析性能, 结果表明该传感器能有效区分互补序列、 单碱基错配序列、 三碱基错配序列和完全错配序列, 可在1.0×10-12~1.0×10-7 mol/L范围内对大肠杆菌目标序列进行定量分析, 检出限为1.3×10-13 mol/L. 将该传感器应用于大肠杆菌实际样品的检测, 结果令人满意.

关键词: DNA生物传感器, CdS纳米空心球, 聚乙烯醇, 电化学交流阻抗

Abstract:

Ahomogeneous CdS-PVA(polyvinyl alcohol) nanocomposite dispersion was obtained by mixing hollow CdS nanospheres in PVA solution under ultrasonic condition. Then the dispersion was dropped on a glassy carbon electrode for dryness to obtain the CdS-PVA modified electrode. Further, a novel DNA biosensor for Escherichia coli was fabricated by covalent immobilization of Escherichia coli gene related oligonucleotides on the modified electrode using terephthalic acid as the arm linker. The analytical performance of the biosensor was investigated by electrochemical impedance method, and the results revealed that the constructed biosensor had a wide dynamic range from 1.0×10-12 mol/L to 1.0×10-7 mol/L and a low detection limit of 1.3×10-13 mol/L. The selectivity experiments showed that the DNA biosensor could accurately discriminate the complementary sequence from the single-base mismatched, three-base mismatched and noncomplementay sequences. When the biosensor was applied for the detection of the real sample of Escherichia coli, a satisfying result was obtained.

Key words: DNA biosensor, Hollow CdS nanospheres, Polyvinyl alcohol, Electrochemical impedance spectroscopy

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