高等学校化学学报

高等学校化学学报

• 研究论文 • 上一篇    

具有聚集诱导发光性质的高灵敏度和高光稳定性的脂滴荧光探针

高鑫1，†，卿佳1,†，胡祎辰2，3，†，上官之春4，梁同玲2，周永胜1,*，张关心2,*，张德清2，3   

  1. 1.北京大学口腔医学院口腔修复科，国家口腔医学中心，口腔疾病国家临床医学研究中心，口腔生物材料与数字诊疗设备国家工程研究中心，颅颌面组织生物智造与修复再生北京市重点实验室，国家卫生健康委员会数字口腔医学重点实验室，国家药品监督管理局口腔材料重点实验室 2.中国科学院化学研究所分子科学国家研究中心，中国科学院有机固体重点实验室 3.中国科学院大学化学科学学院 4.温州大学化学与材料工程学院，温州生物健康材料与化学重点实验室
  • 收稿日期:2025-12-30 修回日期:2026-01-09 网络首发:2026-01-16 发布日期:2026-01-16
  • 通讯作者: 张关心 E-mail: gxzhang@iccas.ac.cn
  • 基金资助:
    国家自然科学基金（批准号：82530030, 8227095, 22021002）资助

Novel AIE Fluorescent Probes for Ultrahigh Sensitivity and High Photostability in Lipid Droplets Imaging

GAO Xin1, †, QING Jia1, †, HU Yichen2,3, †, SHUANGGUAN Zhichun4, LIANG Tongling2, ZHOU Yongsheng1, *, ZHANG Guanxin2, *, ZHANG Deqing2,3   

  1. 1. Department of Prosthodontics, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology & NHC Key Laboratory of Digital Stomatology & NMPA Key Laboratory for Dental Materials 2. Beijing National Laboratory for Molecular Sciences, CAS Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences 3. School of Chemical Sciences, University of Chinese Academy of Sciences 4. College of Chemistry and Materials Engineering, Wenzhou University, Key Lab of Biohealth Materials and Chemistry of Wenzhou
  • Received:2025-12-30 Revised:2026-01-09 Online First:2026-01-16 Published:2026-01-16
  • Contact: Guanxin Zhang E-mail: gxzhang@iccas.ac.cn
  • Supported by:
    Supported by the National Natural Science Foundation of China (Nos. 82530030, 22021002)

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补充材料

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摘要： 我们设计合成了两种新型聚集诱导发光（AIE）探针TPA-H与TPA-2F。这两种探针均表现出优异的生物相容性（浓度高达50 μM时，细胞存活率仍超过90%）和脂滴靶向特异性。在3T3-L1脂肪细胞的早期分化过程中，TPA-2F与TPA-H均能清晰显像BODIPY难以检测的微小以及新生脂滴，显示出更优的成像灵敏度。此外，TPA-2F 展现出高的光稳定性，在连续160次激光扫描后，其荧光强度仍保持初始值的90%以上。本研究不仅提供了两种高性能的脂滴成像工具，更凸显了AIE发光材料在细胞器特异性生物成像中的应用潜力，为脂质相关代谢疾病的早期诊断与机制研究提供了新的可能途径。

关键词: 聚集诱导发光（AIE）, 荧光成像, 脂滴, 光稳定性

Abstract: We designed and synthesized two novel aggregation-induced emission (AIE)-active probes, TPA-H and TPA-2F, based on a triphenylamine (TPA) core. Systematic characterization demonstrated that both probes exhibit excellent biocompatibility (cell viability > 90% at concentrations up to 50 μM) and outstanding LD-targeting specificity with minimal colocalization with other organelles such as mitochondria and lysosomes. During early differentiation of 3T3-L1 adipocytes, both TPA-2F and TPA-H clearly visualized small and nascent LDs that were difficult to detect with BODIPY, indicating superior imaging sensitivity. Moreover, TPA-2F demonstrated exceptional photostability, retaining over 90% of its initial fluorescence intensity after 160 continuous laser scanning cycles, significantly outperforming TPA-H. This work not only provides two high-performance LD imaging tools but also highlights the potential of AIE luminogens (AIEgens) in organelle-specific bioimaging, offering promising avenues for early diagnosis and mechanistic research of lipid-related metabolic diseases.

Key words: aggregation-induced emission (AIE), fluorescence imaging, lipid droplets；photostability

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