高等学校化学学报

高等学校化学学报

• 研究论文 • 上一篇    下一篇

基于构象自适荧光团构建的比率型荧光探针及其脂滴原位可视化研究

周意超1,田贺1,韩海浩2,王辰瀚1,胡习乐1,贺晓鹏1   

  1. 1. 结构可控先进功能材料及其制备教育部重点实验室， 费林加诺贝尔奖科学家联合研究中心， 华东理工大学化学与分子工程学院 2. 烟台新药创制山东省实验室
  • 收稿日期:2025-07-22 修回日期:2025-09-27 出版日期:2025-10-30 发布日期:2025-10-30
  • 通讯作者: 贺晓鹏 E-mail:xphe@ecust.edu.cn
  • 基金资助:
    国家自然科学基金（批准号： 92253306， 82130099， 22477030）资助

Fluorescent probes constructed based on conformationally-adaptive fluorophores for the in-situ visualization of lipid droplets

 ZHOU Yichao1， TIAN He1， HAN Haihao2， WANG Chenhan1， HU XiLe1， HE Xiaopeng1*   

  1. 1. Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering， Feringa Nobel Prize Scientist Joint Research Center， Frontiers Center for Materiobiology and Dynamic Chemistry， School of Chemistry and Molecular Engineering， East China University of Science and Technology 2. Shandong Laboratory of Yantai Drug Discovery， Bohai Rim Advanced Research Institute for Drug Discovery
  • Received:2025-07-22 Revised:2025-09-27 Online:2025-10-30 Published:2025-10-30
  • Contact: Xiao-Peng HE E-mail:xphe@ecust.edu.cn
  • Supported by:
    Supported by the National Nature Science Foundation of China(Nos.92253306， 82130099， 22477030)

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摘要： 本文通过具有双荧光发射特性的“构象自适”荧光基团N,N’-二苯基-二氢二苯并[a，c]吩嗪（DPAC）和线粒体靶向基团三苯基膦（TPP）之间的偶联，构建了可对脂滴（LD）和线粒体双重标记的比率型荧光探针TPP-DPAC. 通过将探针与油酸（OA）诱导胞内LD生成的HepG2细胞共孵育，证实探针能同时对LD和线粒体进行标记，荧光成像共定位系数（Rr）分别为0.96和0.95，进一步实验表明，探针由于对所处细胞微环境的构象自适应，可动态发生红/蓝荧光信号变化，从而实现细胞中的LD形成与融合等动态事件的原位示踪. 该比率型荧光探针为监测细胞内LD代谢过程以及其与线粒体的相互作用提供了可视化工具，也为探究LD与其他亚细胞器的相互作用研究提供了新策略.

关键词: 荧光探针, 脂滴, 线粒体, 荧光成像

Abstract: A ratiometric fluorescent probe TPP-DPAC capable of simultaneously labeling lipid droplets (LD) and mitochondria was constructed by coupling N,N '-diphenyl-dihydrobenzo [a, c] phenazine (DPAC) characteristic of dual-fluorescence emission with a mitochondrial-targeting group triphenylphosphine (TPP). By incubating the probe with HepG2 cells pretreated with oleic acid (OA) for LD production, the simultaneous fluorescence imaging of LDs and mitochondria was achieved applying two emission channels, with a high Pearson’s coefficient of 0.96 and 0.95 when overlapped with commercial LD and mitochondrial tracker, respectively. The fluorescence emission of the probe was shown to be adaptable to the surrounding microenvironment, thus exhibiting a subtle blue-to-red emission change upon the generation of LDs. This unique feature makes it possible to track the generation of LDs and their interactions with mitochondria in real time. This study offers a new chemical tool for the study of LD dynamics and the crosstalk between LDs and other subcellular organelles during various physiological and pathological processes.

Key words: Fluorescent probe, Lipid droplet, Mitochondria, Fluorescence imaging

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