高等学校化学学报 ›› 2012, Vol. 33 ›› Issue (05): 1041-1045.doi: 10.3969/j.issn.0251-0790.2012.05.032

• 物理化学 • 上一篇    下一篇

靶向性纳米金探针对宫颈癌细胞的激光扫描共聚焦散射成像

陈娜1, 秦颂兵2, 张兵波3, 张旭光4, 周菊英2, 涂彧1   

  1. 1. 苏州大学放射医学与防护学院, 苏州 215123;
    2. 苏州大学附属第一人民医院, 苏州 215006;
    3. 同济大学先进材料与纳米生物医学研究院, 上海 200092;
    4. 徐州市肿瘤医院, 徐州 221000
  • 收稿日期:2011-06-09 出版日期:2012-05-10 发布日期:2012-05-10
  • 作者简介:周菊英, 女, 副教授, 主要从事临床放射治疗工作. E-mail: zhjuying@sohu.com
    涂 彧, 男, 教授, 主要从事放射卫生和放射防护研究. E-mail: tuyu@suda.edu.cn
  • 基金资助:

    国家自然科学基金(批准号: 51003078), 同济大学青年优秀人才培养计划项目(批准号: 2009KJ072)和江苏省研究生培养创新工程(批准号: CXLX11-0080)资助.

Scattering Imaging of Cervical Cancer Cell by Laser Scanning Confocal Microscopy with Gold Nano-probes

CHEN Na1, QIN Song-Bing2, ZHANG Bing-Bo3, ZHANG Xu-Guang4, ZHOU Ju-Ying2, TU Yu1   

  1. 1. School of Radiation Medicine and Protection, Soochow University, Suzhou 215123, China;
    2. First Affiliated Hospital of Soochow University, Suzhou 215006, China;
    3. Institute for Advanced Materials & Nano Biomedicine, Tongji University, Shanghai 200092, China;
    4. Xuzhou Cancer Hospital, Xuzhou 221000, China
  • Received:2011-06-09 Online:2012-05-10 Published:2012-05-10

摘要: 制备了粒径均一的纳米金颗粒, 再对其表面进行叶酸修饰, 制得具有靶向性的纳米金探针. 利用激光扫描共聚焦显微镜(LSCM), 对靶向性纳米金的细胞特异性散射成像进行研究. 实验结果表明, 人宫颈癌细胞(Hela)对纳米金-叶酸的摄取作用强于对纳米金的摄取, 但随着时间的延长, 两者的差别逐渐减小. 表明在适当的时间内纳米金-叶酸探针对宫颈癌细胞具有良好的靶向性.

关键词: 纳米金, 叶酸, 激光扫描共聚焦显微镜, 散射成像, 宫颈癌细胞

Abstract: The quantities and activities of folate receptor are higher in tumor cells than those in normal cells, so it has become the ideal ligand for targeted therapy of tumor. Due to the unique optical property of gold nanoparticles(AuNPs), the scattering light from AuNPs can be detected when they are excited by a single laser. Based on these principles, in this study AuNPs with uniform size were prepared, then modified with folic acid(FA), the resulting AuNPs-FA were used as targeting probes for imaging of cervical cancer cells(Hela). The specific scattering imaging could be researched by laser scanning confocal microscopy(LSCM). The results show that the effect of intaking of AuNPs-FA is stronger than that of AuNPs by the cervical cancer cells. However, with the extension of time, the difference will gradually decrease. So the AuNPs-FA has a good targeting property to label the cervical cancer cells in the right time.

Key words: Gold nanoparticles, Folic acid, Laser scanning confocal microscopy(LSCM), Scattering imaging, Cervical cancer cell

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