高等学校化学学报 ›› 2018, Vol. 39 ›› Issue (12): 2665-2672.doi: 10.7503/cjcu20180319

• 分析化学 • 上一篇    下一篇

水溶性超分子荧光探针对多菌灵的识别及细胞成像

杨梅1,2, 刘青2, 唐青4, 王成会2, 杨梅香2, 孙涛3, 黄英1,2(), 陶朱2   

  1. 1. 贵州大学西南药用生物资源教育部工程研究中心
    2. 贵州省大环化学及超分子化学重点实验室
    3. 贵州省高性能计算化学重点实验室
    4. 烟草学院, 贵阳 550025
  • 收稿日期:2018-04-25 出版日期:2018-11-09 发布日期:2018-11-09
  • 作者简介:

    联系人简介: 黄 英, 女, 博士, 教授, 主要从事药物分析化学方面的研究. E-mail: yinghung128@163.com

  • 基金资助:
    国家自然科学基金(批准号: 21562015)、 贵州省优秀青年科技人才培养计划(批准号: 黔教合平台人才[2017]5636)、 贵州省普通高等学校科技拔尖人才支持计划项目(批准号: 黔教合 KY字[2016]059)、 贵州省科技厅自然科学基金重点项目(批准号: 黔教合 JZ字[2014]2005)、 贵州省科技厅联合基金项目(批准号: 黔教合 LH字[2014]7658)和教育部春晖计划合作科研项目(批准号: Z2015002)资助.

Water-soluble Supramolecular Fluorescent Probe for Sensing Carbendazim and Its Application in Living Cell Imaging

YANG Mei1,2, LIU Qing2, TANG Qing4, WANG Chenghui2, YANG Meixiang2, SUN Tao3, HUANG Ying1,2,*(), TAO Zhu2   

  1. 1. The Engineering and Research Center for Southwest Bio-Pharmaceutical Resources of National Education Ministry of China
    2. Key Laboratory of Macrocyclic and Supramolecular Chemistry of Guizhou Province
    3. Key Laboratory of Guizhou High Performance Computational Chemistry
    4. College of Tobacco Science, Guizhou University, Guiyang 550025, China
  • Received:2018-04-25 Online:2018-11-09 Published:2018-11-09
  • Contact: HUANG Ying E-mail:yinghung128@163.com
  • Supported by:
    † Supported by the National Natural Science Foundation of China(No.21562015), the Excellent Youth Scientific Talents Foundation of Guizhou Province, China(No.[2017]5636), the Talents Support Project of Guizhou Province of Education, China(No.QJH-KY-2016-059), the Natural Science Fund of the Science and Technology Department of Guizhou Province, China(No.QJH-JZ-2014-2005), the Mutual Fund Project of the Science and Technology Department of Guizhou Province, China(No.QJH-LH-2014-7658) and the Cooperation Projects of Chunhui Plan of the Ministry of Education of China(No.Z2015002).

摘要:

基于八元瓜环(Q[8])可使吖啶橙(AD)的荧光降低的性质制备了荧光探针2AO@Q[8], 当在该探针中加入多菌灵后荧光强度又逐渐增强, 利用此超分子配合物的荧光效应, 构建了一种能够检测多菌灵的超分子荧光探针. 研究结果表明, 该探针在水溶液中对多菌灵具有良好的选择性, 在一定浓度范围内其线性关系良好, 检出限为8.14×10-8 mol/L. 细胞成像结果显示, 该探针在前列腺癌细胞中对多菌灵具有良好的响应, 可用于生物细胞内多菌灵的识别检测.

关键词: 八元瓜环, 吖啶橙, 荧光探针, 多菌灵, 细胞成像

Abstract:

A host-guest complex between cucurbit[8]uril(Q[8]) and acridine orange(AO) was used as a fluorescent probe for detecting the carbendazim(CBZ). 2AO@Q[8] fluorescent probe was designed and used to detect carbendazim with high sensitivity and selectivity with a detection limit of ca. 10-8 mol/L. A good linear relationship of emission intensity at ca. 529 nm for CBZ at concentrations of 0.4—5.0 μmol/L was observed. The proposed sensing mechanism was investigated via 1H NMR spectroscopy combined with density functional theory calculations at the B3LYP/6-31G(d) level. The cell imaging study showed that the 2AO@Q[8] complex could be used to detect carbendazim in prostate cancer(PC3) cells, which may help to sense carbendazim in biological system.

Key words: Cucurbit[8]uril, Acridine orange, Fluorescent probe, Carbendazim, Cell imaging

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