高等学校化学学报 ›› 2018, Vol. 39 ›› Issue (8): 1640-1646.doi: 10.7503/cjcu20170853

• 分析化学 • 上一篇    下一篇

嵌段聚合物修饰毛细管电色谱柱分离分析解热镇痛药物

冯文雅1,2, 乔娟2,3, 齐莉2,3, 李志伟1()   

  1. 1. 河北科技大学化学与制药工程学院, 石家庄 050018
    2. 中国科学院化学研究所活体分析化学院重点实验室, 北京分子科学国家中心, 北京 100190
    3. 中国科学院大学, 北京 100049
  • 收稿日期:2017-12-28 出版日期:2018-08-10 发布日期:2018-05-12
  • 作者简介:联系人简介: 李志伟, 男, 博士, 副教授, 主要从事药物分析及质量控制方面的研究. E-mail: lizhiwei@hebust.edu.cn
  • 基金资助:
    国家自然科学基金(批准号: 21727809, 21635008, 21621062)和中国科学院前沿科学重点研究项目(批准号: QYZDJ-SSW-SLH034)资助.

Sepatation of Antipyretic Analgesics by CapillaryElectrochromatography with Block Copolymer Coating

FENG Wenya1,2, QIAO Juan2,3, QI Li2,3,*, LI Zhiwei1,*()   

  1. 1. College of Chemical and Pharmaceutical, Heibei University of Science andTechnology, Shijiazhuang 050018, China
    2. Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China
    3. University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2017-12-28 Online:2018-08-10 Published:2018-05-12
  • Contact: QI Li,LI Zhiwei E-mail:lizhiwei@hebust.edu.cn
  • Supported by:
    † Supported by the National Natural Science Foundation of China(Nos.21727809, 21635008, 21621062) and the Key Programs of the Chinese Academy of Sciences, China(No.QYZDJ-SSW-SLH034).

摘要:

采用可逆加成断裂链转移聚合法合成了具有两亲性质的嵌段聚合物聚苯乙烯马来酸酐, 通过共价键合将其涂覆于毛细管内壁, 构建了一种新型开管柱毛细管电色谱(OT-CEC)方法, 并将其用于解热镇痛药物的分离分析. 考察了缓冲溶液pH值、 磷酸盐浓度、 有机溶剂的种类及添加量对3种药物(氨基比林、 非那西汀和4-氨基安替比林)OT-CEC分离效率的影响. 结果表明, 在缓冲溶液中添加15%(体积分数)四氢呋喃后, 修饰于管壁表面的嵌段聚合物可形成胶束结构, 并起到类表面活性剂的作用; 与裸管相比, 该涂层管可明显提高被分析物的分离效率; 连续30次测定电渗流的相对标准偏差<3.0%, 表明该涂层管稳定性较好. 在最佳OT-CEC分析条件下, 对3种药物的分析均获得了良好的定量线性关系(R2>0.988), 检出限为3.5~4.8 μg/mL. 所建立的方法为新型嵌段聚合物涂层的开发及药物的OT-CEC分析奠定了良好基础.

关键词: 开管柱毛细管电色谱, 嵌段聚合物涂层, 解热镇痛药物

Abstract:

A unique open tubular capillary electrochromatography(OT-CEC) system with block copolymer coating was constructed for separation of antipyretic analgesics. The amphipathic poly(maleic anhydride-co-styrene) was synthesized by reversible addition-fragmentation chain transfer polymerization method. The effects of several key factors on the OT-CEC separation efficiency were investigated. Interestingly, by adding organic solvent to the running buffer, the block copolymer coating could self-assemble to form micelle-like spheres and play the role of surfactants. Comparing with the bare capillary, the coating one could enhance the separation efficiency obviously. Moreover, the results indicated that the coating capillaries had a good stability and repeatability. Under the optimized OT-CEC conditions, the developed OT-CEC assay was used to ananlyze the antipyretic drugs in clinical tables with satisfactory linearity(R2≥0.988). The detection limits obtained from the calculations based on signal-to-noise ratio(S/N=3) were 3.5—4.8 μg/mL for the test drugs.

Key words: Open tubular capillary electrochromatography, Block copolymer coating, Antipyretic analgesics

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