高等学校化学学报 ›› 2016, Vol. 37 ›› Issue (12): 2299-2305.doi: 10.7503/cjcu20160513

• 高分子化学 • 上一篇    下一篇

温敏性乙二醇壳聚糖水凝胶的制备及药物缓释性能

李征征1(), 徐子扬1, 高留意1, 曾威1, 赵琳琳2()   

  1. 1. 天津科技大学化工与材料学院, 天津 300457
    2. 天津理工大学材料科学与工程学院, 天津市光电显示材料与器件重点实验室, 天津 300384
  • 收稿日期:2016-07-18 出版日期:2016-12-10 发布日期:2016-10-31
  • 作者简介:联系人简介: 李征征, 女, 博士, 助理研究员, 主要从事生物医用高分子的合成与应用研究. E-mail:li.z.z@tust.edu.cn; 赵琳琳, 男, 博士, 讲师, 主要从事药物载体设计与合成方面的研究. E-mail:luxingzhao@hotmail.com
  • 基金资助:
    天津科技大学校青年创新基金(批准号: 2014CXLG16)资助

Preparation and Characterization of Thermo-sensitive N-Acetyl Glycol Chitosan Hydrogel for Sustained Drug Release

LI Zhengzheng1,*(), XU Ziyang1, GAO Liuyi1, ZENG Wei1, ZHAO Linlin2,*()   

  1. 1. College of Chemical Engineering and Materials Science, Tianjin University of Science and Technology,Tianjin 300457, China
    2. Tianjin Key Laboratory for Photoelectric Materials and Devices,School of Materials Science & Engineering, Tianjin University of Technology, Tianjin 300384, China
  • Received:2016-07-18 Online:2016-12-10 Published:2016-10-31
  • Contact: LI Zhengzheng,ZHAO Linlin E-mail:li.z.z@tust.edu.cn;luxingzhao@hotmail.com
  • Supported by:
    † Supported by the Young Teachers Innovation Fund of Tianjin University of Science and Technology, China(No.2014CXLG16)

摘要:

以乙二醇壳聚糖为原料, 乙酸酐为酰化剂, 通过N-乙酰化反应, 制得了新型温敏性高分子乙酰化乙二醇壳聚糖. 通过核磁共振氢谱(1H NMR)、 傅里叶变换红外光谱(FTIR)及试管倒置法对乙酰化乙二醇壳聚糖的结构及温敏性进行了表征, 通过扫描电子显微镜(SEM)和紫外-可见分光光度计(UV-Vis)对水凝胶的微观形貌和体外药物释放性能进行了研究. 结果表明, 随着反应时间和乙酸酐与乙二醇壳聚糖氨基摩尔比的增加, 产物的乙酰度逐渐增加; 乙酰化乙二醇壳聚糖溶液具有热可逆温敏性溶胶-凝胶转变行为, 可以通过控制乙酰化乙二醇壳聚糖的乙酰度和溶液浓度, 使溶胶-凝胶转变温度处于室温至体温(25~37 ℃)之间; 乙酰化乙二醇壳聚糖水凝胶具有“高度孔隙化且孔隙之间相互连通”的结构特点, 通过控制乙酰度和溶液浓度, 可使其孔径大小处于1~40 μm范围内; 乙酰化乙二醇壳聚糖水凝胶的乙酰度为89.90%时, 质量分数为5%~7%的水凝胶对抗癌药物吉西他滨具有缓释作用, 载药凝胶的释药时间可达3~5 d. 乙酰化乙二醇壳聚糖有望在药物释放及组织工程等领域得到广泛应用.

关键词: 乙二醇壳聚糖, 乙酰化乙二醇壳聚糖, 温敏性水凝胶, 药物缓释载体

Abstract:

A novel thermo-sensitive polymer N-acetyl glycol chitosan(NAGC) by N-acetylation of glycol chitosan with acetic anhydride was prepared. The chemical structure, thermo-sensitive property, morphology and in vitro drug release properties of NAGC hydrogel were investigated by means of 1H NMR, FTIR, tube inverting method, SEM and UV-Vis spectroscopy. The results show that the degree of acetylation(DA) of NAGC is increased along with the increase of the feed molar ratio of acetic anhydride to glycol chitosan and reaction time. NAGC hydrogel shows reversible thermo-sensitive sol-gel transition behavior, and the sol-gel transition temperature is well controlled from 25 ℃ to 37 ℃ by controlling the DA and polymer concentration. NAGC hydrogels show macroporous structure with the pore size of 1—40 μm. The pores are well-interconnected to each other and the pore size could be controlled by changing the DA and concentration of NAGC. NAGC hydrogel(DA=89.90%, 5%—7%, mass fraction) shows sustained release behavior for anticancer drug gemcitabine, and the release time could be controlled from 3 d to 5 d. NAGC shows great promise for various biomedical applications, such as drug delivery and tissue engineering.

Key words: Glycol chitosan, N-Acetyl glycol chitosan, Thermo-sensitive hydrogel, Drug release carrier

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