高等学校化学学报 ›› 2016, Vol. 37 ›› Issue (2): 221.doi: 10.7503/cjcu20150886

• 研究快报 • 上一篇    下一篇

基于宿主细胞中磷脂“自然嵌合”的杆状病毒叶酸修饰

王丽英, 文莉, 吕诚, 林毅(), 庞代文   

  1. 武汉大学生物医学分析化学教育部重点实验室, 化学与分子科学学院,病毒学国家重点实验室, 高等研究院, 武汉 430072
  • 收稿日期:2015-11-19 出版日期:2016-02-10 发布日期:2016-01-06
  • 作者简介:联系人简介: 林 毅, 女, 博士, 副教授, 主要从事表面分析化学及纳米生物技术研究. E-mail:ylin@whu.edu.cn
  • 基金资助:
    国家“九七三”计划项目(批准号: 2011CB933600)、 国家自然科学基金(批准号: 21275111, 21535005)和国家留学基金(批准号: 201406275115)资助

Modification of Baculovirus with Folic Acid based on Phospholipids Self-insertion in Host Cells

WANG Liying, WEN Li, LÜ Cheng, LIN Yi*(), PANG Daiwen   

  1. Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education,College of Chemistry and Molecular Sciences, State Key Laboratory of Virology, the Institute for Advanced Studies, Wuhan University, Wuhan 430072, China
  • Received:2015-11-19 Online:2016-02-10 Published:2016-01-06
  • Contact: LIN Yi E-mail:ylin@whu.edu.cn
  • Supported by:
    † Supported by the National Basic Research Program of China(No.2011CB933600), the National Natural Science Foundation of China(Nos.21275111, 21535005) and the China Scholarship Council(No.201406275115)

关键词: 杆状病毒, 叶酸, 修饰, 磷脂, 宿主细胞

Abstract:

Baculovirus has the advantages such as outstanding biosafety, large cloning capacity and non-replication in the transduced cells. However, their in vivo applications are hampered for lacking of specificity. Therefore, modification of baculovirus with targeting molecules is urgently needed. Herein, a method is proposed for the modification of folic acid(FA) on baculovirus based on phospho-lipids self-insertionin host cells. Immunofluorescent colocalization and transmission electron microscope(TEM) results showed that the modified baculovirus kept their integrity while maintaining their infectivity. Thus the method offers opportunities for the development of tumor-targeting gene vectors and vaccines.

Key words: Baculovirus, Folic acid, Modification, Lipid, Host cell

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