高等学校化学学报 ›› 2016, Vol. 37 ›› Issue (4): 643.doi: 10.7503/cjcu20150725

• 分析化学 • 上一篇    下一篇

用于检测细胞内谷胱甘肽的比率型荧光探针

杨润洁, 唐尧, 朱维平()   

  1. 华东理工大学药学院, 上海市化学生物学重点实验室, 上海 200237
  • 收稿日期:2015-09-17 出版日期:2016-04-10 发布日期:2016-03-10
  • 基金资助:
    国家自然科学基金(批准号: 21476077)资助

Ratiometric Fluorescent Probe for the Detection of Glutathione in Living Cells

YANG Runjie, TANG Yao, ZHU Weiping*()   

  1. Shanghai Key Lab of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China
  • Received:2015-09-17 Online:2016-04-10 Published:2016-03-10
  • Contact: ZHU Weiping E-mail:wpzhu@ecust.edu.cn
  • Supported by:
    † Supported by the National Natural Science Foundation of China(No.21476077)

摘要:

设计合成了1种用于检测生物巯基的比率型荧光探针(4), 并考察了其对谷胱甘肽的识别作用. 在4-羟乙基哌嗪乙磺酸(HEPES)缓冲液中, 探针4可与谷胱甘肽快速反应, 溶液颜色由淡黄色变为粉红色, 从而实现“裸眼”检测, 且在608 nm处的荧光信号增强. 在1.6×10-5~2×10-4 mol/L范围内, 探针4能够定量检测谷胱甘肽, 检出限为8.9×10-7 mol/L. 此外, 探针4还可用于MCF-7细胞中谷胱甘肽的成像.

关键词: 荧光探针, 谷胱甘肽, 细胞成像

Abstract:

A fluorescent probe(4) for the detection of biothiols was designed and synthesized and its properties for labeling glutathione was investigated. After reaction with glutathione in 2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid(HEPES) buffer, the color of solution changed from light yellow to pink, which could be detected by naked eyes. Meanwhile, the fluorescence was enhanced at 608 nm. Probe 4 was useful for measuring glutathione at concentrations ranging from 1.6×10-5 to 2×10-4 mol/L with a detection limit of 8.9×10-7 mol/L. Besides, probe 4 was sensitive to glutathione in MCF-7 cells, which emitted red fluorescence when it was incubated with MCF-7 cells.

Key words: Fluorescent probe, Glutathione, Intracellular imaging

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