高等学校化学学报 ›› 2014, Vol. 35 ›› Issue (6): 1311.doi: 10.7503/cjcu20131185

• 物理化学 • 上一篇    下一篇

基于弹性网络模型的麦芽糖转运蛋白功能残基的识别

周晓峰1, 谢小露2, 张蕾2, 李春华2(), 楼姣英3, 刘乃波1, 丁振山1()   

  1. 1.中日友好医院泌尿外科, 北京 100029
    2. 北京工业大学生命科学与生物工程学院, 北京 100124
    3. 北京中医药大学东方医院, 北京 100078
  • 收稿日期:2013-12-05 出版日期:2014-06-10 发布日期:2014-04-29
  • 作者简介:联系人简介: 李春华, 女, 博士, 教授, 主要从事计算分子生物学与生物信息学研究. E-mail:chunhuali@bjut.edu.cn;丁振山, 男, 博士, 主治医师, 主要从事肿瘤治疗与药物设计研究. E-mail:dzsfighting@sina.com
  • 基金资助:
    国家自然科学基金(批准号: 31171267)、 北京市教委项目(批准号: KM201310005030)、 北京市教委科技创新平台项目和吴阶平医学基金(批准号: 320.6750.12703) 资助

Idenpngication of Functional Residues in Maltose Transporter with the Elastic Network Model

ZHOU Xiaofeng1, XIE Xiaolu2, ZHANG Lei2, LI Chunhua2,*(), LOU Jiaoying3, LIU Naibo1, DING Zhenshan1,*()   

  1. 1. Department of Urology, China-Japan Friendship Hospital, Beijing 100029, China
    2. College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, China
    3. Dong Fang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China
  • Received:2013-12-05 Online:2014-06-10 Published:2014-04-29
  • Contact: LI Chunhua,DING Zhenshan E-mail:chunhuali@bjut.edu.cn;dzsfighting@sina.com
  • Supported by:
    † Supported by the National Natural Science Foundation of China(No.31171267), the Project of Beijing Municipal Education Commission, China(No.KM201310005030), the Science and Technology Innovation Platform of Beijing Municipal Education Commission and Wu Jie Ping Medical Foundation, China(No.320.6750.12703)

摘要:

基于弹性网络模型的热力学方法, 识别出麦芽糖转运蛋白质体系中的关键残基, 探讨了麦芽糖转运蛋白内长程协同效应, 研究结果有助于更好地理解该转运体系发挥生物学功能的分子机制.

关键词: 麦芽糖转运蛋白, 弹性网络模型, 热力学循环, 变构效应, 功能残基

Abstract:

Maltose transporter is an important kind of transmembrane transporters with significant biological functions which bears the transportation of intracellular and extracellular maltose molecules. The transport process of maltose molecule accompanies with large scale cooperative motions between different domains of maltose transporter. And these motions involve some key residues that mediate signal transduction and allosteric effects between different regions of the protein. In this study, we idenpngied the key residues in this protein system with the thermodynamic method based on the elastic network model which was developed by our group. On this basis, we explored the long-range synergies within the maltose transporter. These results are helpful to a better understanding the molecular mechanisms of function of transporters.

Key words: Maltose transporter, Elastic network model, Thermodynamic cycle, Allosteric effect, Functional residue

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