Preparation of Potentiometric Enzyme Electrode for Urea Based on Enzyme Immobilization by 60% Quaternized Poly(4-vinyl)pyridine

Abstract

Abstract: Based on electrostatic interaction between 60% quaternized poly(4-vinyl)pyridine and urease molecules, which can be immobilized on the surface of pH glassy electrode. By means of this technique, the enzyme electrode for urea was prepared. In experiments, we discussed in detail effects of kinds, concentration and pHof media on response of the electrode. In 0.025mol/Lphosphate buffer solution(pH 6.8),the linearity range is between 1×10^-4 and 6×10^-2 mol/Land the detection limit is 1×10^-4 mol/L. The electrode has been applied to the determination of urea in standard serum and recovery is between 92.9% and 107.0%.

Key words: Enzyme electrode, Urea, Potentiometry

CLC Number:

O657.15

TrendMD:

LI Wen-Jiang, LIU Xiao-Ya, SUN Chang-Qing  . Preparation of Potentiometric Enzyme Electrode for Urea Based on Enzyme Immobilization by 60% Quaternized Poly(4-vinyl)pyridine[J]. Chem. J. Chinese Universities, 1998, 19(5): 711.

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Preparation of Potentiometric Enzyme Electrode for Urea Based on Enzyme Immobilization by 60% Quaternized Poly(4-vinyl)pyridine

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