Microgravimetric Amplification and the Detection of Short DNA Sequences on a Quartz Crystal Microbalance by Modified Nano-microspheres

Abstract

Abstract: Anovel and sensitive microgravimetric biosensor has been developed by using actinomycin Dfunctionalized magnetic nano-microspheres, which can be intercalated in duplex strands DNAs on the gold electrode of a quartz crystal microbalance. These modified nano-microspheres have been used as the amplified label for microgravimetric signal owing to their heavy mass. The linear range for the determination of target DNAis from 10^-9 to 10^-11 mol/L, and the detection limit is found to be 2×10^-12mol/L.

Key words: Quartz crystal microbalance, Biosensor, Actinomycin D, Magnetic microsphere, Self-assembly monolayer film

CLC Number:

O654.1

TrendMD:

ZHANG Sheng-Long, PENG Tu-Zhi . Microgravimetric Amplification and the Detection of Short DNA Sequences on a Quartz Crystal Microbalance by Modified Nano-microspheres[J]. Chem. J. Chinese Universities, 2002, 23(6): 1022.

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Microgravimetric Amplification and the Detection of Short DNA Sequences on a Quartz Crystal Microbalance by Modified Nano-microspheres

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