Chem. J. Chinese Universities ›› 2011, Vol. 32 ›› Issue (11): 2521.

• Articles • Previous Articles     Next Articles

NMR-based Metabonomics Studies on Serum and Urine of Yang-deficiency Constitution

LI Ying-Shuai, WANG Qi*, YUAN Zhuo-Jun   

  1. Basic Medicine College, Beijing University of Chinese Medicine, Beijing 100029, China
  • Received:2010-12-03 Revised:2011-02-25 Online:2011-11-10 Published:2011-10-14
  • Contact: WANG Qi E-mail:wangqi710@126.com
  • Supported by:

    国家自然科学基金(批准号: 90709050)资助.

Abstract: Nuclear magnetic resonance(NMR)-based metabonomics was applied to study the yang-deficiency constitution by analyzing metabolic profiling of serum and urine. The purpose of this study is to determine an array of characteristic metabolites in serum and urine samples from yang-deficiency constitution person and then to interpret these metabolites in possible metabolic pathway.  Serum and urine samples from 30 yang-deficiency constitution person with 30 balanced constitution person were collected. Differences in endogenous metabolites were detected on serum and urine samples from the yang-deficiency constitution group and control group using multivariate statistical analysis. Compared with the balanced constitution, the contents of lactate, very low density lipoprotein(VLDL)/low density lipoprotein(LDL), N-cetyl glycoprotein(NAc), fatty acid(FA), unsaturated fatty acid(UFA) are lower while the contents of glutamine, glucose, phosphatidylcholine(PtdCho), high density lipoprotein(HDL) are higher in serum of yang-deficiency constitution, and the content of creatinine is lower while the contents of lactate, DMA, citrate, hippurate are higher in urine of yang-deficiency constitution. The biomarkers supply new ways for the individual differences from the point of view of metabonomics. Yang-deficiency constitution has some metabolism disorder of energy, lipid and glucose, and also  has changes of organs functions.

Key words: Yang-deficiency constitution, Nuclear magnetic resonance(NMR), Metabonomics

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