Detection of Cocaine Based on the System of AIEgen, Aptamer and Exonuclease Ⅰ †

doi:10.7503/cjcu20190681

Abstract

Abstract:

A new system for detection of cocaine was designed based on an AIEgen of TPE-TTA, anticocaine aptamer and exonuclease Ⅰ(Exo Ⅰ) . The Exo Ⅰ can specifically cleave the phosphodiester bonds of nucleotides in nucleic acids either from the ends or interior of single stranded DNA(ssDNA). Meanwhile, the cocaine can combine with anticocaine aptamer and switch its configuration to Y-shaped double stranded DNAs(dsDNA) that prevent the aptamer degradation in the presence of Exo Ⅰ. When cationic 4,4',4″,4″'-{[ethene-1,1,2,2-tetrayltetrakis(benzene-4,1-diyl)]tetrakis(oxy)}-tetrakis(N,N,N-triethylbutan-1-aminium) bromide(TPE-TTA) was added to the Tris-HCl solution, it can combine with aptamer and the fluore-scence was turned on. The experiment conditions, including the amount of Exo Ⅰ, incubation time of the Exo Ⅰ with anticocaine aptamer, incubation temperature and time of anticocaine aptamer with cocaine were systematically investigated and the optimal conditions were obtained. With different concentrations of cocaine, the experimental results showed that the fluorescence intensity was progressively enhanced. The limit of detection(LOD) of 0.66 μmol/L was deduced. Moreover, the developed method also was used to determine cocaine in real urine samples, and the LOD can reach as low as 1.0 μmol/L. This work provides a new method for cocaine detection, which is promising for practical application.

Key words: Aggregation-induced emission, Cocaine, Aptamer, Exonuclease Ⅰ

CLC Number:

TrendMD:

DU Xianchao, HAO Hongxia, QIN Anjun, TANG Benzhong. Detection of Cocaine Based on the System of AIEgen, Aptamer and Exonuclease Ⅰ ^†[J]. Chem. J. Chinese Universities, 2020, 41(3): 411.

Figures/Tables 5

Scheme 1 Schematic illustration of detection of cocaine by the system of AIEgen, aptamers and exonuclease Ⅰ

Scheme 2 Synthetic routes of TPE-TTA

Fig.1 Photoluminescence(PL) spectra of TPE-TTA in Tris-HCl solution upon addition of anticocaine aptamer from 0 to 0.2 μmol/L(A) and exonuclease-mixture treated and untreated anticocaine aptamer in the presence or absence of cocaine(B) λex=340 nm; c(TPE-TTA)=5 μmol/L. (B) c(anticocaine aptamer)=0.2 μmol/L.

Fig.2 PL spectra of anticocaine aptamer/TPE-TTA in buffer with addition of Exo Ⅰ(A) and plots of relative PL intensity of sensing system versus incubation time of Exo Ⅰ with anticocaine aptamer(B), cocaine incubation temperature with anticocaine aptamer(C) and incubation time of anticocaine aptamer with cocaine(D) λex=340 nm, c(anticocaine aptamer)=0.2 μmol/L; c(TPE-TTA)=5 μmol/L.

Fig.3 PL spectra of sensing system upon addition of cocaine in buffer solution(A) and the plot of relative intensity versus cocaine concentration(B) Inset of (B): the plot with cocaine concentration from 0 to 100 μmol/L. λex=340 nm; c(anticocaine aptamer)=0.2 μmol/L; c(TPE-TTA)=5 μmol/L.

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