Specific Hepatocellular Carcinoma Imaging Based on Aggregation-Induced Emission Nanoparticles †

doi:10.7503/cjcu20190398

Abstract

Abstract:

A bright orange fluorescent nanoparticles NDSA@SALL4[NDSA=4,4'-(1E,1'E)-2,2-(anthracene-9,10-diyl)bis(ethane-2,1-diyl)bis(N,N-dimethylaniline), SALL4=Sal-like protein 4] encapsulated by amphiphilic polymer 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[succinimidyl(polyethylene glycol)](DSPE-PEG-NHS) was prepared, which further modified SALL4 antibody. The nanoparticles NDSA@SALL4 shows good biocompatibility and high photoluminescence, which peaked at 559 nm and exhibited a fluorescence quantum yield of 2.89 % in aqueous medium. NDSA@SALL4 can not only specifically target liver cancer cells, but also accurately image the nucleus of cell with bright orange fluorescence, demonstrating that they have a good candidate for early diagnose and bioimaging applications.

Key words: Aggregation-induced emission, Sal-like protein 4(SALL4), Fluorescence bioimaging

CLC Number:

O631

TrendMD:

ZHANG Yu, JING Jiangbo, SHAO Yueming, YIN Xin, XU Bin, WEN Xiaoyu. Specific Hepatocellular Carcinoma Imaging Based on Aggregation-Induced Emission Nanoparticles ^†[J]. Chem. J. Chinese Universities, 2019, 40(11): 2382.

Figures/Tables 6

Scheme 1 Synthetic routes of NDSA

Scheme 2 Preparation of NDSA nanoparticles

Fig.1 Emission spectra(A) of NDSA in DCM/hexane mixtures with different hexane volume fractions and plot of relative emission intensity(I/I0) versus the composition of the DCM/hexane mixture of NDSA(B)

Fig.2 Size distribution of NDSA NPs without(A) and with(B) SALL4 antibody modification The insets are TEM images.

Fig.3 Normalized UV-Vis absorption and PL emission spectra(excitation wavelength: 365 nm) of NDSA@SALL4 in water solution The inset is the picture of NDSA NPs in water solution under the room light(A) and UV-light(B), respectively.

Fig.4 Confocal imaging of HepG2 cells(A—D), HL-7702 cells(E—H) after incubation with 50 mg/L NDSA NPs and blank Hep-G2 cells(I—L) (A), (E), (I) Bright; (B), (F), (J) DAPI; (C), (G), (K) NPs; (D), (H), (L) merge.

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