Tuneable Three-dimensional Cytocompatible Micro-environments Designed by Controlled Alignment of PVDF Electrospun Fibres

doi:10.7503/cjcu20160832

Abstract

Abstract:

Three dimensional(3D) aligned electrospun fibres have a promising potential application in biomedical areas, such as biosensors, controlled drug release and tissue engineering. However, the fabrication of these fibres with tuneable microarchitectures, porosity and overall morphology still has challenges due to electrospinning process. Thus, it is highly desired to develop novel, cost-effective and easily scalable fabrication methods for the 3D aligned electrospun fibres. Herein, we developed a facile yet effective method for the preparation of 3D poly(vinylidene fluoride)(PVDF) aligned fibres(3D AFs) via an improved electrospinning technique. The obtained 3D AFs showed controllable morphology, diameter and fiber density. Furthermore, the obtained 3D AFs showed excellent in vitro biocompatibility. Moreover, the as-prepared 3D AFs enhanced cellular activities and induced directional cell growth along the direction of nanofibre orientation, thereby providing an excellent cue for the anchorage and migration of human mesenchymal stem cells(hMSCs). More importantly, cell proliferation in the 3D AFs was found to be significantly higher than that on the nanofibre mats(NFMs). Combined with controllable morphology and structure, we anticipate that this finding greatly enhances the potential applications of these 3D AFs for therapeutically relevant 3D cell cultures, tissue engineering, diagnostics and other biomedical applications.

Key words: Electrospinning, Poly(vinylidene fluoride)(PVDF), Human mesenchymal stem cell(hMSC), Tissue engingeering

CLC Number:

O632.21

TrendMD:

JIN Lin, XU Qinwei, HU Bin, HUANG Jingbin, ZHANG Yilei, WANG Zhenling. Tuneable Three-dimensional Cytocompatible Micro-environments Designed by Controlled Alignment of PVDF Electrospun Fibres[J]. Chem. J. Chinese Universities, 2017, 38(6): 1002.

Figures/Tables 7

Fig.1 Illustration of the improved electrospinning process with the special co-axial frisbee disc collectors arranged on a rotary shaft for the fabrication of 3D aligned nanofibres(A), photographic image of the aligned 3D oriented nanofibres(B), SEM images of a traditional PVDF aligned fibre mesh(2D AFs, C) and 3D fluffy PVDF aligned fibres(3D AFs, D)

Fig.2 BET surface area of 2D random nanofibres, 2D aligned nanofibres and 3D aligned nanofibres

Fig.3 SEM images of 3D aligned nanofibres showing differences in nanofiber diameters(A1—C1) and the nanofiber diameter distribution within the scaffolds(A2—C2) with respect to PVDF concentrationPVDF concentration(%): (A) 13; (B) 16; (C) 18.

Fig.4 SEM images of 3D aligned nanofibres with different densitiesCollecting time/min: (A) 30; (B) 60.

Fig.5 SEM images(day 5) of hMSCs cultured on the 2D random nanofibres(A), 2D aligned nanofibres(B) and 3D aligned nanofibres(C)

Fig.6 Fluorescence images of hMSCs cultured for 5 d on/in 2D random nanofibres(A), 2D aligned nanofibres(B) and 3D aligned nanofibres(C)

Fig.7 Proliferation of hMSCs cultured for various periods on/in 2D random nanofibres, 2D aligned nanofibres and 3D aligned nanofibres

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