Structural Characterization of Purified Polysaccharide Fraction(PLP-1) from the Seeds of Plantago asiatica L.

doi:10.7503/cjcu20130382

Abstract

Abstract:

Polysaccharide's biological activities are related to their structure. Previous studies showed that the polysaccharide from seeds of Plantago asiatica L. had good bioactivities, and two fractions from the crude polysaccharide were characterized. This work aimed at investigating primary structure of another fraction, PLP-1. The methods, including monosaccharide compositions analysis, partial acid hydrolysis, nuclear magnetic resonance(NMR), gas chromatography(GC) and gas chromatography-mass spectroscopy(GC-MS), were applied for structural characterization. The results showed that PLP-1 was composed of Ara(33.98%), Xyl(58.23%), Rha(2.36%), Glc(2.23%), Gal(2.36%) and Man(0.83%). Its main uronic acid was GlcA. The majority residues were α-T-linked Araf(9.58%), β-T-linked Xylp(8.71%), α-1,3-linked Araf(18.22%), β-1,3-linked Xylp(8.05%), β-1,4-linked Xylp(5.85%), β-1,2,4-linked Xylp(16.98%) and β-1,3,4-linked Xylp(27.52%). GlcA was existed as α-T-linked GlcAp. There were other residues, including 1,2-linked Rhap, T-linked Glcp, 1,6-linked Glcp, T-linked Galp, 1,3-linked Galp and 1,3,6-linked Galp, etc. Finally, primary structure of PLP-1 was proposed.

Key words: Seeds of Plantago asiatica L., Polysaccharide, Primary Structure, Methylation

CLC Number:

O629.12

TrendMD:

YIN Jun-Yi, LI Chang, NIE Shao-Ping, XIE Ming-Yong. Structural Characterization of Purified Polysaccharide Fraction(PLP-1) from the Seeds of Plantago asiatica L.[J]. Chem. J. Chinese Universities, 2013, 34(12): 2728.

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