Abstract:

In order to study the impact of extracellular matrix material on endothelial cells, collagen, poly-lysine and fusion protein VEGF-Fc were immobilized on the polystyrene cell culture plate by physical adsorbing, respectively. The three proteins significantly improved the hydrophilic of the polystyrene surface. Meanwhile, the cell adhesion, proliferation and the expressions of the cytoskeletal protein and von Willebrand factor(vWF) were investigated. The results showed that these matrixes effectively improved the adhesion of Human umbilical vein endothelial cells(HUVECs). And HUVECs cultured on fusion protein VEGF-Fc matrix without extra VEGF in the culture medium could also maintain and prolong the bioactivities of cell proliferation and vWF expression. Furthermore, HUVECs cultured on collagen and fusion protein matrixes expressed abundant stress fibers and filopodia. Thereby, the collagen and fusion protein VEGF-Fc matrixes constructed in this work provided suitable environment for HUVECs. Those results will provide a theoretical basis on achieving the endothelialization and improving the angiogenesis of implanted materials.

Key words: Polystyrene, Fusion protein, Tissue engineering, Physical adsorption, Vascularization