Electrochemistry and Electrocatalysis of Hemoglobin with SiO2 Gel Film Layer-by-layer Assembled on Carbon Nanotubes-Au Nanoparticles Modified Electrode

Abstract

Abstract: Protein direct electrochemistry study and its electro\|catalysis analysis were introduced, involving layer-by-layer alternate assembly of hemoglobin(Hb)and SiO2 gel film onto electrode surface. The electrode was firstly modified with the nano-compound of multi-walled carbon nanotubes and Au nanoparticles, i.e., MWNTs-Au/GC electrode. Then Hb and SiO2 gel film were alternately spread onto the electrode surface to stabilize the protein and its amount and bioactivity, thus resulting {SiO2/Hb}n/MWNTs-Au/GC electrode. Under the optimized n=2, the Hb on the {SiO2/Hb}2/MWNTs-Au/GC electrode displayed its intrinsic bioactivity well, transferring electrodes with electrode and catalyzing H2O2 reduction as one peroxidaze enzyme.

Key words: Hemoglobin, Carbon nanotube, Au nanoparticle, SiO2 gel

CLC Number:

O657.1

TrendMD:

XU Ying, ZHANG Xiao-Yan, YANG Jing*, HE Pin-Gang*, FANG Yu-Zhi. Electrochemistry and Electrocatalysis of Hemoglobin with SiO2 Gel Film Layer-by-layer Assembled on Carbon Nanotubes-Au Nanoparticles Modified Electrode[J]. Chem. J. Chinese Universities, 2011, 32(10): 2293.

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