Abstract: One-step extraction method was studied for the rapid extraction of pure celluloses from the corn stalk, in acetic acid-nitric acid mixture under 10 different reaction conditions, and then the extracted cellulo-sic samples were hydrolyzed. The experimental results show that the optimized parameters for isolation of celluloses are reaction temperature at 120 ℃, 1∶25 of the solid-liquid ratio, 80% acetic acid and 10% nitric acid, reaction duration for 20 min, and the yield of cellulose is 38%. Hydrolysis of cellulosic samples show that the content of glucose is above 90% in preparations obtained under the optimized condition, and hydrolysis rate arrived to 94%. 13C NMR and FTIR indicate that the structure of cellulose is not damaged, and the content of cellulose Iβ is the highest, and the high degree of both lignin and hemicellulose degradation and removal is achieved in acetic acid-nitric acid mixture. The result shows a remarkable potential for pure cellulose production.

Key words: Cellulose, One-step extraction, Nitric acid, Hydrolysis