Chem. J. Chinese Universities ›› 2010, Vol. 31 ›› Issue (9): 1884.

• Articles • Previous Articles     Next Articles

Adsorption of Protein on Conducting Poly(methacryloyl pyrrole)

ZHANG Zhi-Hong*, YAN Li-Jun, LIANG Yan, FENG Xiao-Zhong, YAN Fu-Feng, ZHENG Xian-Jun, WANG Li-Zhen   

  1. Henan Provincial Key Laboratory of Surface & Interface Science, Zhengzhou University of Light Industry, Zhengzhou 450002, China
  • Received:2009-11-27 Online:2010-09-10 Published:2010-09-10
  • Contact: ZHANG Zhi-Hong. E-mail: mainzhh@yahoo.com.cn
  • Supported by:

    国家自然科学基金 (批准号: 20704039)、河南省高校科技创新人才支持计划(批准号: 2010HASTIT023)和河南省国际科技合作计划(批准号: 094300510078)资助.

Abstract: Poly(methacryloyl pyrrole) films were prepared by surface initiated atom transfer radical polymerization(SIATRP) onto the surface of silicon and Au. The chemical structure, the surface wettability, the surface morphology and the electrochemical property were investgated. The adsorption behavior of BSA on the polymer surface was measured in situ by surface plasmon resonance spectroscopy. The adsorption mechanism of BSA could be revealed based on the effects of buffer pH values and BSA concentration on the protein adsorption. The change in the chemical property after BSA immobilization was characterized by cyclic voltammetry. The morphologies of PMAP before and after BSA adsorption were investigated by atom force microscopy. This study could supply the base of the preparation of the regular, uniform and sensitive films which are used in the field of biosensors.

Key words: Surface initiated atom transfer radical polymerization(SIATRP), Bovine serum albumin(BSA), Surfae plamson resonane spectrscopy(SPR), Polypyrrole

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