Identified of Urinary Modified Nucleosides of Lymph Cancer Patients by HPLC/ESI-Q-TOF MS

Abstract

Abstract:

As modified nucleosides reflect altered tRNA turnover which is impaired in the body of cancer patients, they have been evaluated as potential tumor markers. High performance liquid chromatography(HPLC) was combined with full-scan mass spectrometry, MS/MS analysis and accurate mass measurements in order to identify modified nucleosides which purified from urine. Potential urinary nucleosides were assessed by their evident UV absorbance in the HPLC chromatogram and then further examined by the mass spectrometric techniques. In this manner the information of 9 nucleosides were reproduced with the standard compounds and 17 modified nucleosides without commercial standards were identified in the urine samples from lymph cancer patients including 6 novel urinary modified nucleosides: N3-methyladenosine, N7-methyladenine, 5′-dehydro-2′-deoxyinosine, N3-methylguanine, O6-methylguanosine and N7-methyl-N1-ethylguanosine. This new method is rapid and specific to identify the complex biological samples without control compounds.

Key words: HPLC/ESI-Q-TOF MS, Modified nucleosides, Lymph cancer, Urine

CLC Number:

O657.6

TrendMD:

LI Hua-Yu, LIU Hong-Min*, BU Shan-Shan, LI Juan, HAN Dong, WU Guang-Yin, WANG Shao-Min, ZHANG Ming-Zhi. Identified of Urinary Modified Nucleosides of Lymph Cancer Patients by HPLC/ESI-Q-TOF MS[J]. Chem. J. Chinese Universities, 2009, 30(4): 661.

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