On-line Isolation of Lysozyme from Egg-white Using Multi-walled Carbon Nanotubes

Chem. J. Chinese Universities

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On-line Isolation of Lysozyme from Egg-white Using Multi-walled Carbon Nanotubes

DU Zhuo, ZHANG Na, WANG Jian-Hua*

Research Center for Analytical Sciences, Northeastern University, Shenyang 110004, China

Received:2007-10-30 Revised:1900-01-01 Online:2008-05-10 Published:2008-05-10
Contact: WANG Jian-Hua

Abstract

Abstract: A novel procedure for the extraction of lysozyme from egg-white using carbon nanotubes as a sorbent was developed. Carbon nanotubes after oxidation and purification were used to pack a micro-column which was incorporated into a sequential injection system for facilitating online selective sorption of lysozyme from egg-white. The retained protein was afterwards quantitatively eluted by a carbonate buffer. With a sample loading volume of 2.0 mL, an enrichment factor of 12 was obtained, along with a retention efficiency of 100% and a recovery rate of 100%. A sampling frequency of 10 h^-1 was achieved along with a precision of 3.0% RSD. Lysozyme of 0.4 g could be extracted from 100 mL egg-white. SDS-PAGE demonstrated that the purity of the obtained lysozyme was as high as that of pure reagent.

Key words: Carbon nanotube, Solid phase extraction, Lysozyme, Egg-white

CLC Number:

O657

TrendMD:

DU Zhuo, ZHANG Na, WANG Jian-Hua*. On-line Isolation of Lysozyme from Egg-white Using Multi-walled Carbon Nanotubes[J]. Chem. J. Chinese Universities, doi: .

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On-line Isolation of Lysozyme from Egg-white Using Multi-walled Carbon Nanotubes

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