Fluorescence Cross-correlation Spectroscopy Using Single Wavelength Laser Excitation

Chem. J. Chinese Universities

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Fluorescence Cross-correlation Spectroscopy Using Single Wavelength Laser Excitation

XIE Chao, DONG Chao-Qing, REN Ji-Cun*

College of Chemistry and Chemical Engineering, Shanghai Jiaotong University, Shanghai 200240, China

Received:2007-11-06 Revised:1900-01-01 Online:2008-05-10 Published:2008-05-10
Contact: REN Ji-Cun

Abstract

Abstract: The basic principle of fluorescence cross-correlation spectroscopy were introduced in this article. A setup of fluorescence cross-correlation spectroscopy(FCCS) was established with single wavelength laser. After the optimization of the setup, the detection volume is about 0.7 fL. This home-built setup was successfully applied for study of the binding reaction of human immunoglobulin G with goat anti-human immunoglobulin G. With quantum dots(745 nm) and Rhodamine B(580 nm) as probes and 532 nm laser beam as excitation source, the cross-talk effect was near completely suppressed. The molecule numbers in a highly focused volume, concentration, diffusion time and hydrodynamic radius of the reaction product can be determined by FCCS system.

Key words: Fluorescence cross-correlation spectroscopy, Single-molecule detection, Single wavelength laser excitation, Quantum dot

CLC Number:

O657

TrendMD:

XIE Chao, DONG Chao-Qing, REN Ji-Cun*. Fluorescence Cross-correlation Spectroscopy Using Single Wavelength Laser Excitation[J]. Chem. J. Chinese Universities, doi: .

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Fluorescence Cross-correlation Spectroscopy Using Single Wavelength Laser Excitation

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