Purification and Properties of Recombinant Ganoderma Lucidum Immunoregulatory Protein

Chem. J. Chinese Universities

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Purification and Properties of Recombinant Ganoderma Lucidum Immunoregulatory Protein

ZHU Jian-Qiang², LIANG Chong-Yang¹, FENG Kai², GAI Xiao-Dong², SUN Xin², SUN Fei¹*

1. Institute of Regenerative Medical Science, School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China;
2. Center of Life Science, Beihua University, Jilin 132013, China

Received:2007-07-09 Revised:1900-01-01 Online:2008-04-10 Published:2008-04-10
Contact: SUN Fei

Abstract

Abstract: Recombinant Ganoderma lucidum immunoregulatory protein(rGlip) was purified from fermentation. Purification was carried out by ultrafiltration, anion exchange chromatography(AIEC), hydrophobic interaction chromatography(HIC), size exclusion chromatography(SEC). We used Hitrap Q sepharose column to measure the optimal pH value of anion exchange chromatography and detect the absorption value of protein at 215, 254, 280 nm. The intensity absorption of rGlip was found at 215 nm. Hemagglutination reactionno aggregation was observed between any types of human red blood cells, the positive activity was seen in the presence of sheep blood red cells. The optimal pH of AIEC enhanced the yield of initial purification, HIC was designed for lyophobic domains of rGlip, and end product(rGlip) of purification procedures possessed aggregation activity of sheep red blood cells.

Key words: Ganoderma lucidum, Anion exchange chromatography, Hydrophobic interaction chromatography, Size exclusion chromatography, Purification

CLC Number:

Q539
O629

TrendMD:

ZHU Jian-Qiang², LIANG Chong-Yang¹, FENG Kai², GAI Xiao-Dong², SUN Xin², SUN Fei¹*. Purification and Properties of Recombinant Ganoderma Lucidum Immunoregulatory Protein[J]. Chem. J. Chinese Universities, doi: .

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Purification and Properties of Recombinant Ganoderma Lucidum Immunoregulatory Protein

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