Extraction, Separation, Structural Analysis and Antioxidant Activity in vitro of Arabinoxylans(AX-Ⅰ-1) from the Residue of Astragalus Root†

doi:10.7503/cjcu20160500

Abstract

Abstract:

Four fractions(AX-Ⅰ-1, AX-Ⅰ-2, AX-Ⅰ-3 and AX-Ⅰ-4)from the residue of Astragalus root were extracted by sequential chemical extraction, and purified by DEAE-Cellulose 52 anion-exchange and Sepharcryl S-400 high resolution gel-permeation chromatography. A combination of chemical and instrumental analysis was performed to investigate the structural characterization and antioxidant activity of AX-Ⅰ-1. The results demonstrated that AX-Ⅰ-1 was mainly composed of rhamnose, arabinose, xylose, mannose, glucose and galactose, and the molar ratio was 0.006∶14.113∶8.284∶0.116∶0.468∶1. Backbone of AX-Ⅰ-1 composed of arabinose and xylose by β-(1→2), β-(1→3)and β-(1→4)glycosidic bond. The branches were composed of (1→4)-linked-βArap, (1→3)-linked-βGalp, (1→2)-linked-βManp, and the terminal residues were αRhap, βGclp, βGalp.The four fractions had a certain scavenging effect on hydroxyl radical, superoxide anion radical and DPPH radical. When the concentration is 0.1 mg/mL, scavenging rate of AX-Ⅰ-2, AX-Ⅰ-3 and AX-Ⅰ-4 on superoxide anion is about 2 times higher than the positive control vitamin C.

Key words: Residue of astragalus root, Arabinoxylan, Purification, Structural analysis, Antioxidant activity

TrendMD:

LIU Lei, LI Ke, HAO Xia, WANG Guizhen, QIN Xuemei, DU Guanhua, ZHANG Xiang. Extraction, Separation, Structural Analysis and Antioxidant Activity in vitro of Arabinoxylans(AX-Ⅰ-1) from the Residue of Astragalus Root^†[J]. Chem. J. Chinese Universities, 2016, 37(12): 2168.

Figures/Tables 9

References 30

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Sugar	Methylated sugar	m/z	Molar ratio	Linkages type
Rha-	2,3,4-Me₃-Rha	59, 72, 85, 102, 113, 118, 131, 162, 175	0.01	L-Rhap-(1→
Ara-	2-Me-Ara	59, 74, 85, 99, 118, 127, 141, 159, 173, 187, 201, 217, 261	13.42	→3,4)-L-Arap-(1→
Xyl-	2-Me-Xyl	61, 73, 86, 115, 128, 145, 159, 175, 188, 218, 243, 261, 289	8.13	→2,3,4)-D-Xylp-(1→
Man-	3,4,6-Me₃-Man	60, 71, 87, 99, 117, 129, 141, 161, 171, 189	0.10	→2)-D-Manp-(1→
Glc-	2,3,4,6-Me₄-Glc	59, 71, 87, 102, 118, 129, 145, 162, 175, 205	0.24	D-Glcp-(1→
	4-Me-Glc	59, 71, 87, 100, 116, 129, 143, 160, 171, 189, 201, 262	0.23	→2,3,6)-D-Glcp-(1→
Gal-	4,6-Me₂-Gal	59, 71, 86, 101, 112, 129, 143, 161, 188, 202	0.48	→2,3)-D-Galp-(1→
	3,4,6-Me₃-Gal	59, 71, 88, 100, 113, 119, 129, 145, 161, 174, 190	0.49	→2)-D-Galp-(1→

Sugar	Methylated sugar	m/z	Molar ratio	Linkages type
Rha-	2,3,4-Me₃-Rha	59, 72, 85, 102, 113, 118, 131, 162, 175	0.01	L-Rhap-(1→
Ara-	2-Me-Ara	59, 74, 85, 99, 118, 127, 141, 159, 173, 187, 201, 217, 261	13.42	→3,4)-L-Arap-(1→
Xyl-	2-Me-Xyl	61, 73, 86, 115, 128, 145, 159, 175, 188, 218, 243, 261, 289	8.13	→2,3,4)-D-Xylp-(1→
Man-	3,4,6-Me₃-Man	60, 71, 87, 99, 117, 129, 141, 161, 171, 189	0.10	→2)-D-Manp-(1→
Glc-	2,3,4,6-Me₄-Glc	59, 71, 87, 102, 118, 129, 145, 162, 175, 205	0.24	D-Glcp-(1→
	4-Me-Glc	59, 71, 87, 100, 116, 129, 143, 160, 171, 189, 201, 262	0.23	→2,3,6)-D-Glcp-(1→
Gal-	4,6-Me₂-Gal	59, 71, 86, 101, 112, 129, 143, 161, 188, 202	0.48	→2,3)-D-Galp-(1→
	3,4,6-Me₃-Gal	59, 71, 88, 100, 113, 119, 129, 145, 161, 174, 190	0.49	→2)-D-Galp-(1→

Residue	δ
Residue	1-H/C1	2-H/C2	3-H/C3	4-H/C4	5-H/C5	6-H/C6
α-L-Rhap(1→ (A)	5.02/102.4	4.34/70.9	3.99/81.0	3.44/72.0	3.83/69.5	1.24/
→3,4)β-L-Arap(1→(B)	4.26/102.2	4.22/75.9	3.87/83.8	3.99/82.1	3.82/63.7	-/-
→2,3,4)β-D-Xylp(1→(C)	4.76/107.0	3.07/74.4	3.44/73.1	3.82/81.6	3.36/59.6	-/-
→2)β-D-Manp(1→(D)	4.91/94.6	3.36/80.9	3.25/75.9	3.87/73.1	3.82/70.0	3.71/63.7
β-D-Glcp(1→ (E)	4.87/99.6	3.44/73.1	3.82/74.4	3.66/66.9	3.71/70.1	3.72/59.6
→2,3,6)α-D-Glcp(1→(F)	5.01/99.8	3.42/81.0	3.66/73.1	3.71/75.4	3.82/70.0	3.87/73.1
→2,3)β-D-Galp(1→(G)	4.84/102.4	3.82/69.3	3.99/82.6	4.04/70.0	3.66/75.9	3.71/59.6
→2)β-D-Galp(1→(H)	4.82/102.0	3.72/70.0	3.87/73.6	3.89/71.8	3.44/74.8	3.72/60.5

Residue	δ
Residue	1-H/C1	2-H/C2	3-H/C3	4-H/C4	5-H/C5	6-H/C6
α-L-Rhap(1→ (A)	5.02/102.4	4.34/70.9	3.99/81.0	3.44/72.0	3.83/69.5	1.24/
→3,4)β-L-Arap(1→(B)	4.26/102.2	4.22/75.9	3.87/83.8	3.99/82.1	3.82/63.7	-/-
→2,3,4)β-D-Xylp(1→(C)	4.76/107.0	3.07/74.4	3.44/73.1	3.82/81.6	3.36/59.6	-/-
→2)β-D-Manp(1→(D)	4.91/94.6	3.36/80.9	3.25/75.9	3.87/73.1	3.82/70.0	3.71/63.7
β-D-Glcp(1→ (E)	4.87/99.6	3.44/73.1	3.82/74.4	3.66/66.9	3.71/70.1	3.72/59.6
→2,3,6)α-D-Glcp(1→(F)	5.01/99.8	3.42/81.0	3.66/73.1	3.71/75.4	3.82/70.0	3.87/73.1
→2,3)β-D-Galp(1→(G)	4.84/102.4	3.82/69.3	3.99/82.6	4.04/70.0	3.66/75.9	3.71/59.6
→2)β-D-Galp(1→(H)	4.82/102.0	3.72/70.0	3.87/73.6	3.89/71.8	3.44/74.8	3.72/60.5