Polypeptide from Chlamys Farreri Inhibits UVA-induced Apoptosis of HaCaT Cells Involving of aSMase-JNK Pathway

Chem. J. Chinese Universities

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Polypeptide from Chlamys Farreri Inhibits UVA-induced Apoptosis of HaCaT Cells Involving of aSMase-JNK Pathway

XING Yan-Xia^1,2, SU Ai³, DU Wei³, WANG Chun-Bo⁴*

1. Department of Biochemistry and Molecular Biology, Medical College, Qingdao University, Qingdao 266071, China;
2. Department of Biochemistry, Medical College, Shanxi Datong University, Datong 037008, China;
3. Laboratory of Cellular and Molecular Biology,
4. Department of Pharmacology, Medical College, Qingdao University, Qingdao 266071, China

Received:2007-07-17 Revised:1900-01-01 Online:2008-04-10 Published:2008-04-10
Contact: WANG Chun-Bo

Abstract

Abstract: A pathological model of UVA-induced HaCaT cells was established to investigate whether polypeptide from Chlamys farreri(PCF) protects HaCaT cells from apoptosis induced by UVA through acid sphingomyelinase(aSMase)-JNK pathway and to explore its related molecular mechanism. Apoptosis of cells were determined by Hoechst 33258 staining and agarose gel electrophoresis. The expression of aSMase in HaCaT cells was detected via RT-PCR and immunofluorescence. Using Western Blot analysis, expression levels of JNK and phosphorylated JNK were assayed. The results showed that PCF can significantly protect against UVA-induced apoptosis of HaCaT cells. PCF can also inhibit expression of aSMase and phosphorylated of JNK in HaCaT cells radiated by UVA in a dose-dependent manner. ASMase inhibitor Desipramine and JNK inhibitor SP600125 had inhibitory effects on UVA-induced apoptosis, the former blocked phosphorylated of JNK. So it is concluded that PCF can protect HaCaT cells from apoptosis induced by UVA and its protective effects may attribute to blocking aSMase-JNK pathway.

Key words: Polypeptide from Chlamys farreri(PCF), Acid sphingomyelinase(aSMase), JNK, Ultraviolet A, Apoptosis, HaCaT cell

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XING Yan-Xia^1,2, SU Ai³, DU Wei³, WANG Chun-Bo⁴*. Polypeptide from Chlamys Farreri Inhibits UVA-induced Apoptosis of HaCaT Cells Involving of aSMase-JNK Pathway[J]. Chem. J. Chinese Universities, doi: .

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Polypeptide from Chlamys Farreri Inhibits UVA-induced Apoptosis of HaCaT Cells Involving of aSMase-JNK Pathway

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