Active Peptide from Acetes Chinensis with Inhibitory Activity on Neuraminidase of Influenza Virus

doi:10.7503/cjcu20130334

Abstract

Abstract:

Low-valued Acetes Chinensis was chosen to prepare the enzymatic hydrolysate with neuraminidase(NA) inhibitory activity by controlled hydrolysis. A novel NA inhibitory peptide was purified by gel chromatography and reversed phase high performance liquid chromatography. The IC₅₀ value of the peptide was only 96.1 μmol/L and it could also significantly inhibit influenza A virus replication in MDCK cells. The amino acid sequence of this peptide was EISYIHAEAYRRGELK which identified by LC-MS/MS and its binding with NA protein was proved by UV spectrum analysis. The accurate sites of NA protein that the peptide bind to were determined using the computer assisted software SYBYL. The largest non-toxic concentration(TC₀) was 1.26 mg/mL which assayed by cell cytotoxicity experiments. The hemagglutination essay showed that this pepetide could obviously decrease the agglutination titer influenza virus in a dose-dependent manner which suggested that the antiviral activity of this peptide might be attributed to its action on multiple targets.

Key words: Acetes Chinensis, Neuraminidase, NA inhibitory peptide, Isolation and purification, NA binding site

CLC Number:

O629.72

TrendMD:

WANG Hai-Tao, WANG Wei, CHEN Ming, YUAN Ning, ZHAO Yuan-Hui, MAO Xiang-Zhao. Active Peptide from Acetes Chinensis with Inhibitory Activity on Neuraminidase of Influenza Virus[J]. Chem. J. Chinese Universities, 2013, 34(11): 2540.

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