Properties of Poly[L-(aspartic acid)-co-L-lysine] Grafted with Cholesterol Conjugated Low Molecular Weight Poly(ethylenimine) for Gene Delivery

Chem. J. Chinese Universities

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Properties of Poly[L-(aspartic acid)-co-L-lysine] Grafted with Cholesterol Conjugated Low Molecular Weight Poly(ethylenimine) for Gene Delivery

WANG Yan-Ming, ZHANG Zhen-Fang, YU Qi, WANG Lian-Yong, KONG De-Ling^*, YU Yao-Ting

Key Laboratory of Bioactive Materials of Ministry of Education, College of Life Science, Nankai University, Tianjin 300071, China

Received:2007-11-29 Revised:1900-01-01 Online:2008-10-10 Published:2008-10-10
Contact: KONG De-Ling

Abstract

Abstract: A new type of tumor-targeted non-viral gene vector, PSL-PEI600-Chol, was synthesized by ring-opening of poly[succinimide-co-lysine](PSL) with low-molecular weight poly(ethylenimine)(PEI600) and its cholesterol derivatives, respectively. The important parameters for preparation of PSL-PEI600-Chol/DNA complexes and characterization of physico-chemical properties of this delivery system were also investigated. It is indicated that PSL-PEI600-Chol could form compact complexes with DNA at a mass ratio higher than 5. The transfection efficiencies of PSL-PEI600-Chol were evaluated in different cell lines and the results showed that cholesterol modification could enhance the delivery of pEGFP-C1 into tumor cells HepG2 and Hela. These results suggest that with a lower cytotoxicity, higher gene delivery capacity and versatility for chemical conjugation, PSL-PEI600-Chol could warrant further investigation for a promising nonviral gene vector.

Key words: Poly[L-(aspartic acid)-co-L-lysine], Cholesterol, Poly(ethylenimine), Gene carrier

CLC Number:

Q782
O631.3

TrendMD:

WANG Yan-Ming, ZHANG Zhen-Fang, YU Qi, WANG Lian-Yong, KONG De-Ling^*, YU Yao-Ting. Properties of Poly[L-(aspartic acid)-co-L-lysine] Grafted with Cholesterol Conjugated Low Molecular Weight Poly(ethylenimine) for Gene Delivery[J]. Chem. J. Chinese Universities, doi: .

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Properties of Poly[L-(aspartic acid)-co-L-lysine] Grafted with Cholesterol Conjugated Low Molecular Weight Poly(ethylenimine) for Gene Delivery

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