Determination of Vancomycin in Injection via Enzyme Catalysis-Fluorescence Quenching Method

Chem. J. Chinese Universities

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Determination of Vancomycin in Injection via Enzyme Catalysis-Fluorescence Quenching Method

FENG Xia-Guang, ZHANG Min, ZHAO Hu, WANG Huai-You*

College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, China

Received:2006-11-24 Revised:1900-01-01 Online:2007-07-10 Published:2007-07-10
Contact: WANG Huai-You

Abstract

Abstract:

A new method for determining vancomycin was established based on the principle of fluorescence quenching. The mechanism of the fluorescence quenching was discussed. A linear relationship is obtained between F₀/F and the concentration of vancomycin in the range of 40.0—450.0 μg/mL with the correlation coefficiency 0.9991.The detection limit was 10.91 μg/mL and the relative standard derivative was 0.70%. The effects of pH, foreign ions, standing time on the determination of vancomycin were examined. The results obtained by this method were agreed with those by the official method.

Key words: Vancomycin, Tyrosine, Horseradish peroxidase, H₂O₂, Fluorescence quenching

CLC Number:

O657.39

TrendMD:

FENG Xia-Guang, ZHANG Min, ZHAO Hu, WANG Huai-You*. Determination of Vancomycin in Injection via Enzyme Catalysis-Fluorescence Quenching Method[J]. Chem. J. Chinese Universities, doi: .

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Determination of Vancomycin in Injection via Enzyme Catalysis-Fluorescence Quenching Method

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