Turn-off Fluorescence of Gold Nanoclusters for Sensing of Ferric Ion†

doi:10.7503/cjcu20180369

Abstract

Abstract:

D-Tryptophan-stabilized gold nanoclusters(D-Trp@AuNCs) were prepared by a simple one-pot synthesis protocol and displayed a specific response to Fe³⁺. The resultant AuNCs exhibited a strong blue fluorescence with the maximum emission at 440 nm wavelength. Due to the aggregation-induced fluorescence quenching mechanism, appearance of Fe³⁺ led dramatic fluorescent intensity decrease of AuNCs rather than other cations. Detection limit of the proposed assay for Fe³⁺ was 33.1 nmol/L, with a good linear range of 0.3—500.0 μmol/L(R²=0.9997). D-Trp@AuNCs were further applied in monitoring Fe³⁺ in real water samples, providing a new way for fabrication of AuNCs based fluorescent probes and showing great potential in environmental analysis.

Key words: Gold nanoclusters, D-Tryptophan, Fluorescence quenching, Ferric ion

CLC Number:

O657.8

TrendMD:

LIU Qianrong,CAI Huiwu,GUAN Ming,QIAO Juan,QI Li. Turn-off Fluorescence of Gold Nanoclusters for Sensing of Ferric Ion^†[J]. Chem. J. Chinese Universities, 2019, 40(1): 55.

Figures/Tables 11

Fig.1 FTIR spectra of D-Trp(a) and D-Trp@AuNCs(b)(A) and UV-Vis absorption spectra of D-Trp(a), HAuCl4(b) and D-Trp@AuNCs(c)(B)

Fig.2 Fluorescence excitation(a), emission spectra of fresh D-Trp@AuNCs(b) and stored for a month(c)(A), TEM image(B) and DLS analysis(C) of D-Trp@AuNCsInset of (A): photographs of D-Trp@AuNCs under irradiation of daylight(left) and UV light(right).

Fig.3 Enlarged ESI mass spectra of D-Trp@AuNCs from m/z 820—850(A) and the expanded views of the peaks originated from [Au3Y4+Na10+K-H9]2+(m/z 833.5245)(B)

Fig.4 Effects of reaction time(A), concentrations of D-Trp(B) and HAuCl4(C) on fluorescent intensity of D-Trp@AuNCs

Fig.5 Fluorescence spectra of D-Trp@AuNCs(a) and D-Trp@AuNCs+Fe3+(b)(A), TEM image of D-Trp@AuNCs+Fe3+(B) and DLS analysis of D-Trp@AuNCs+Fe3+(C)Inset of (A): under irradiation of UV light, photographs of D-Trp@AuNCs(left) and D-Trp@AuNCs + Fe3+(right).

Scheme 1 Schematic illustration of Fe3+-mediated fluorescence quenching of D-Trp@AuNCs

Fig.6 Fluorescence spectra of D-Trp@AuNCs(a), D-Trp@AuNCs+Fe3+(10.0 mmol/L) in the presence of 10.0 mmol/L EDTA(b) and D-Trp@AuNCs in the presence of 10.0 mmol/L Fe3+ (c)

Fig.7 Effects of incubation time(A) and buffer pH values(B) on relative fluorescent intensity of Fe3+-D-Trp@AuNCs

Fig.8 Competitive selectivity of D-Trp@AuNCs based sensing probe for Fe3+ over other cations(10.0 mmol/L cations + 10.0 mmol/L Fe3+)

Fig.9 Dose-response graph revealing the fluorescence quenching of D-Trp@AuNCs by Fe3+

Table 1 Determination of Fe3+ in real water samples(n=6)*

Sample	Detected/ (μmol·L^-1)	Spiked/ (μmol·L^-1)	Found/(μmol·L^-1)	Recovery(%)	RSD(%)
Lake water	——	50.0	48.8	97.6	5.4
	——	70.0	68.9	98.4	2.3
	——	120.0	112.6	93.8	3.5
Pond water	——	50.0	43.3	86.6	4.5
	——	70.0	63.8	91.1	5.6
	——	120.0	105.4	87.8	2.6
Tap water	——	50.0	49.3	98.6	3.8
	——	70.0	69.9	99.8	4.5
	——	120.0	127.8	106.5	5.6

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