Ionization Rate and Absolute Intensity of Peptides Enhanced with Matrix-assisted Laser Desorption/Ionization in the Presence of Proteins

Chem. J. Chinese Universities

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Ionization Rate and Absolute Intensity of Peptides Enhanced with Matrix-assisted Laser Desorption/Ionization in the Presence of Proteins

ZHUO Hui-Qin^1,2, HUANG He-Qing^1,2*, WENG Lu-Na^1,2, HUANG Hui-Ying^1,2

1. Key Laboratory of the MOD for Cell Biology and Tumor Cell Engineering, School of Life Sciences,
2. The Key Laboratory of Chemical Biology of Fujian Province, College Chemistry & Chemical Engineering, Xiamen University, Xiamen 361005, China

Received:2006-04-24 Revised:1900-01-01 Online:2007-05-10 Published:2007-05-10
Contact: HUANG He-Qing

Abstract

Abstract: Human serum transferrin(HTF), bovine serum albumin(BSA), and horse spleen ferritin(HSF) have capacities of assisting the matrix to enhance ionization rate and absolute intensity both insulin(INS) and aplysia acidic peptide(AP) by laser desorption/ionization, and this enhancement can be up to 10 and 4 times, respectively. This phenomenon was independent on the concentration of INS, but dependent on these intensifiers strongly. Moreover, the similar enhancing rate of absolute intensity of INS was observed by both SZLF- and apoSZLF-assisted matrix, indicating that this effect was not relative to the metal ions of proteins, but their compositions and structures. The fragment numbers of HTF lysised with endopeptidease and matching rate with reference transferrin were greatly improved in the presence of SZLF, apoSZLF and HSF, when HTF was identified by a combined techniques, lysis enzyme and peptide mass fingerprinting(PMF). This novel effect might play an important role in enhancing the reliability for identifying protein.

Key words: MALDI-TOF MS, Peptide, Absolute intensity, Protein-assisted matrix, PMF

CLC Number:

O629.72
Q51

TrendMD:

ZHUO Hui-Qin^1,2, HUANG He-Qing^1,2*, WENG Lu-Na^1,2, HUANG Hui-Ying^1,2. Ionization Rate and Absolute Intensity of Peptides Enhanced with Matrix-assisted Laser Desorption/Ionization in the Presence of Proteins[J]. Chem. J. Chinese Universities, doi: .

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Ionization Rate and Absolute Intensity of Peptides Enhanced with Matrix-assisted Laser Desorption/Ionization in the Presence of Proteins

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