Separation and Analysis of Constituents of Acidic Compounds in Tobacco Leaf by Using Comprehensive Two-dimensional Gas Chromatography/Time-of-Flight Mass Spectrometry

Chem. J. Chinese Universities ›› 2006, Vol. 27 ›› Issue (4): 612.

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Separation and Analysis of Constituents of Acidic Compounds in Tobacco Leaf by Using Comprehensive Two-dimensional Gas Chromatography/Time-of-Flight Mass Spectrometry

LI Hai-Feng ^1,3, LU Xin¹, LU Hong-Liang ², MA Chen-Fei¹, ZHU Shu-Kui¹, KONG Hong-Wei¹,ZHAO Ming-Yue², XIE Jian-Ping², YE Yao-Rui¹, NIU Sen³, XU Guo-Wang¹*

1. National Chromatographic R & A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; 2. Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450000, China; 3. Plant Protection Department, Shenyang Agriculture University, Shenyang 110161, China

Received:2005-07-04 Online:2006-04-10 Published:2006-04-10
Contact: XU Guo-Wang, E-mail: xugw@dicp.ac.cn

Abstract

Abstract:

Comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry(GC×GC-TOFMS) was applied to the characterization of volatile and semi-volatile acidic compounds in oriental tobacco leaf. Simultaneous distillation extraction(SDE) was used as the sample extraction method. Two types of column sets were compared, and appropriate GC×GC separation conditions were suggested. The separation behavior on two-dimensimal plot of different kinds of compounds were investigated. TOFMS library search combining with structured chromatogram of GC×GC was used to identify the acidic compounds in tobacco leaf. A total of 143 acidic compounds were tentatively identified, including 10 acid anhydrides, 43 organic acids and 90 phenolic compounds. The results show that GC×GC-TOFMS method is suitable for separation and identification of complex sample.

Key words: Tobacco leaf; Acidic compounds; Comprehensive two dimensional gas chromatography(GC×GC); Time of flight mass spectrometry(TOFMS)

CLC Number:

O657

TrendMD:

LI Hai-Feng, LU Xin, LU Hong-Liang, MA Chen-Fei, ZHU Shu-Kui, KONG Hong . Separation and Analysis of Constituents of Acidic Compounds in Tobacco Leaf by Using Comprehensive Two-dimensional Gas Chromatography/Time-of-Flight Mass Spectrometry[J]. Chem. J. Chinese Universities, 2006, 27(4): 612.

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Separation and Analysis of Constituents of Acidic Compounds in Tobacco Leaf by Using Comprehensive Two-dimensional Gas Chromatography/Time-of-Flight Mass Spectrometry

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