Sample Pretreatment Microfluidic Chip for DNA Extraction from Rat Peripheral Blood

Chem. J. Chinese Universities ›› 2006, Vol. 27 ›› Issue (4): 618.

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Sample Pretreatment Microfluidic Chip for DNA Extraction from Rat Peripheral Blood

CHEN Xing¹, CUI Da-Fu ¹*, LIU Chang-Chun¹, LI Hui¹, ZHAO Wei-Xing²

1. State Key Lab of Transducer Tech., Institute of Electronics, Chinese Academy of Sciences, Beijing 100080, China; 2. State
Key Laboratory of Protein and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China

Received:2005-06-20 Online:2006-04-10 Published:2006-04-10
Contact: CUI Da-Fu, dfcui@mail.ie.ac.cn

Abstract

Abstract:

A sample pretreatment microfluidic chip was described based on the principle of solid phase extraction(SPE) and the MEMS technology. Oxidized porous silicon with a large surface area as the solid phase matrix for absorption DNA from biologic sample can greatly improve the DNA yield. The factors which can affect the DNA yield were analyzed and the preparation technology and the experiment procedure were improved. The DNA purification process from the rat peripheral blood can be achieved and the DNA yield is 24 ng/(μL whole blood), which can reach the level of the commercial DNA purification kits. Furthermore, the DNA extracted from the whole blood can be amplified by PCR, which can achieve a high efficiency of the amplification.

Key words: MEMS; Oxidized porous silicon; Sample pretreatment microfluidic chip

CLC Number:

O657
TP212.2

TrendMD:

CHEN Xing, CUI Da-Fu, LIU Chang-Chun, LI Hui, ZHAO Wei-Xing. Sample Pretreatment Microfluidic Chip for DNA Extraction from Rat Peripheral Blood[J]. Chem. J. Chinese Universities, 2006, 27(4): 618.

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Sample Pretreatment Microfluidic Chip for DNA Extraction from Rat Peripheral Blood

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