Tagging Cells with Quantum Dots by Electroporation

Abstract

Abstract: An electroporation method was proposed to make QDs pass through the membranes of K562 leukaemia cells. This method is operated simply and facilitates the tagging experiment greatly. The results show that when the electroporation voltage is 200 V and the lasting time is 1 ms, a high tagging rate is reached. A lot of cells can be tagged at the same time and the livability of the cells is 60%. The whole process can be completed in 20 min. This method brings forward some more extrusive advantages than cell endocytosis and microinjection method, and there should have a very good foreground for further applications.

Key words: Quantum dot, Cell tagging, Electroporation

CLC Number:

O657

TrendMD:

YANG Rui, JI Wei, YAN Yu-Xi, SHI Yan-Yu, FEI Qiang, MU Ying, JIN Qin-Han. Tagging Cells with Quantum Dots by Electroporation[J]. Chem. J. Chinese Universities, 2005, 26(6): 1043.

References

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