Abstract: Chemiluminescence(CL) reaction of manganese(Ⅳ)-formaldehyde-analgin was carried out. Molecular imprinted polymer of analgin was prepared by using methacrylic acid as the functional monomer and ethylene glycol dimethacrylate as the cross-linker. The binding characteristic of the analgin-imprinted polymer was evaluated by Scatchard analysis. A new chemiluminescence method for the determination of analgin was proposed by the combination of analgin-imprinted polymer and manganese(Ⅳ)-formaldehyde-analgin CL system. The linear range is 1.0×10^-7 -1.0×10^-5 mol/L, the detection limit is 4×10^-8 mol/L, the relative standard deviation is 2.4(1.0×10^-6 mol/L analgin, n=7 ). The method was applied to the determination of analgin in human urine samples directly.

Key words: Chemiluminescence, Molecular imprinting, Analgin, Manganese(Ⅳ)