Electrochemical Detection of DNA Sequences Using Nano-magnetic Particles

Abstract

Abstract: The carboxyl nanoparticles were prepared by dispersing polymerization method. The oligonucleotides were immobilized on the surface of the nano-magnetic particles by EDC and NHS. The novel DNA probe of magnetic particle can concentrate the trace amount of complementary DNA selectively from the sample solution onto the gold film electrode under a magnetic field. Differential pulse voltammetry was used to detect DNA with an electroactive indicator of neutral red which could bind to double stranded DNA. The linear range for the determination of target DNA is from 1.0×10^-6 to 5.0×10^-9 mol/L, and the detection limit is found to be 8.6×10^-10 mol/L.

Key words: Nano-magnetic particles, DNA sequence, Concentration, Neutral red

CLC Number:

O657

TrendMD:

LIU Ai-Li, PENG Tu-Zhi, CHENG Qiong. Electrochemical Detection of DNA Sequences Using Nano-magnetic Particles[J]. Chem. J. Chinese Universities, 2005, 26(2): 231.

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Electrochemical Detection of DNA Sequences Using Nano-magnetic Particles

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