Lipase-catalyzed Synthesis of Isoamyl Isobutyrate in Reversed Micelle System

Abstract

Abstract: A new method of lipase-catalyzed esterification of isoamyl isobutyrate by CCL in the reversed micelle of CTAB/n-hexane and AOT/n-hexane has been reported. The effects of such factors as water content(w₀), ratio of substrate to enzyme, pH of buffer and temperature on the synthesis of esterification were investigated. Experimental results show that the reaction rate of esterification in the system of CTAB/n-hexane is faster than that in the system of AOT/n-hexane, and that the conversion of esterification reaches a climax of 99% in the two systems in 80 h. Both the two kinds of reversed micelle provide a suitable micro-environment for the selected lipase(CCL), which shows superactivity 6 and 4 times as much as that in organic solvent, respectively.

Key words: Reversed micelle, Lipase, Isoamyl isobutyrate

CLC Number:

O647

TrendMD:

WANG Yuan-Hong, CHU Ying, LIU Jing-Lin, Lv Zhong-Xian, LIU Ying-Ying, WU Zi-Sheng . Lipase-catalyzed Synthesis of Isoamyl Isobutyrate in Reversed Micelle System[J]. Chem. J. Chinese Universities, 2004, 25(9): 1684.

References

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