Activation of Esterification Activity of Rhizopus Chinensis Lipase in Organic Media by Surfactant†

doi:10.7503/cjcu20180148

Abstract

Abstract:

Rhizopus chinensis lipase(RCL) was co-lyophilized with surfactants and/or inorganic salts to improve the esterification activity in organic solvent. Effects of type and concentration of surfactants and/or inorganic salts on esterification activity were investigated. The results demonstrate that n-dodecyl-β-D-maltoside(DDM) can enhance esterification activity of RCL effectively. The critical factor for activation of non-aqueous activity is molar ratio of DDM to RCL, rather than concentration of DDM. Esterification activity cannot be activated until the molar ratio is greater than 30, and then increase with the ratio. Furthermore, addition of appropriate inorganic salts in medium can strengthen the DDM-induced activation. The DDM-activated RCLs were used to catalyze the synthesis of ethyl hexanoate in organic media and ethyl oleate in solvent-free system. Higher reaction rate and conversion were obtained, which suggests the applicability of this method.

Key words: Surfactant, Lipase, Esterification activity, Non-aqueous catalysis, Inorganic salts

TrendMD:

YANG Yihan,WANG Dong,ZHANG Zhang,XU Yan. Activation of Esterification Activity of Rhizopus Chinensis Lipase in Organic Media by Surfactant^†[J]. Chem. J. Chinese Universities, 2018, 39(9): 1948.

Figures/Tables 5

Fig.1 Effects of different surfactants on the esterification activity of RCL a. Control; b. CTAB; c. OG; d. DDM; e. Tween80; f. TritonX-100.

Fig.2 Effects of the amount of DDM on the esterification activity of RCL with different concentrations

Fig.3 Effects of concentration of different salts on the esterification activity of RCL

Fig.4 Effects of concentration of different salts on the esterification activity of DDM-activated RCL

Fig.5 Time course of synthesis of ethyl hexanoate(A) and ethyl oleate(B) catalyzed by DDM-activated RCLs

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