A Biosensor for Hydrogen Peroxide Based on Peroxidase Immobilized by Plasma Polymerized Film

Abstract

Abstract: A biosensor for hydrogen peroxide assay based on glassy-carbon electrode with plasma polymerized polyethylene diamine film was proposed. Plasma polymerization technique was utilized to make amino groups deposit on the surface of glassy-carbon electrode for an oriented covalent attachment of HRP. The conditions of plasma polymerization including applied power for polymerization, pressure, plasma exposure time and flow rates were studied. The effects of experimental variables such as the operating potential of the working electrode, mediator concentration and pH of the measured solution were investigated for the optimum analytical performance by using an amperometric method, and the plasma polymerized polyethylene diamine film was examined by Fourier transform infrared spectroscopy. The biosensor provided a linear response to hydrogen peroxide over a concentration range of 5×10^-7—1.1×10^-3 mol/L and a detection limit of 0.3 μmol/L. The recovery studies for H₂O₂ in the real samples gave satisfactory results.

Key words: Miccrowave plasma polymerization, Hydrogen peroxide, Horseradish peroxidase(HRP), Biosensor

CLC Number:

O657

TrendMD:

LIU Bing, YANG Ming-Hui, YANG Hai-Feng, SHEN Guo-Li, YU Ru-Qin . A Biosensor for Hydrogen Peroxide Based on Peroxidase Immobilized by Plasma Polymerized Film[J]. Chem. J. Chinese Universities, 2004, 25(10): 1820.

References

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