Studies on the Method of Flow Cytometry Integrated with Microfluidic Chip

Abstract

Abstract: A microfluidic chip is designed and fabricated, and coupled with flow cytometry. By using Na₂HPO₄ solution added with hydroxypropyl methyl cellulose(HPMC) as buffer and sheathing fluid, the problems met with the flowing of microspheres in the microchannel were solved, and the fluidic conditions were under more effective control. By using a home-made laser induced fluorescence system and electrokinetic focusing, the counting of the microspheres and real time observation of their actual fluidic state through an inverted fluorescence microscope is made. The whole system is compact and easy to be operated. The comsumption of reagent and sample is little and the speed of the assay is quick.

Key words: Microfluidic chip, Fluorescence microspheres, Flow cytometry, Electrokinetic focusing, Adsorption

CLC Number:

O657

TrendMD:

YANG Rui, MU Ying, ZOU Ming-Qiang, WU Zhe, JIN Qin-Han . Studies on the Method of Flow Cytometry Integrated with Microfluidic Chip[J]. Chem. J. Chinese Universities, 2004, 25(10): 1816.

References

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