Studies on the Interaction of Daunomycin and the Specific Sequence of DNA

Abstract

Abstract: On the ground of UV-spectrophotometry and UV-spectroelectrochemistry, the interaction of daunomycin(DNR) and specific sequence of DNAhas been investigated.As the results obtained in this paper, DNRcan react with the sequence of oligonucleotide which is CpG, ApGand ApCsuccessively; the sequence of base pairs of double helix of DNAwhich are intercalated by DNRare (CpG)₂, (TpG)(CpA) and (CpT)(ApG).An important conclusion has been gained in this paper that if Gbases were not pairing in the helix of DNA, the spectroelectrochemical characteristic of the adducts containing that kind of DNAand DNRwould exhibit a remarkable difference with those adducts consisting of DNRand normal DNA.It indicates that this characteristic can be used to detect and determine the DNAhelix which has one base unpaired or mispairing.The results obtained in this paper are of clinical and pharmacaLImportance.

Key words: Daunomycin, Anathracycline, DNA Speciality, Spectroelectrochemistry

CLC Number:

O657

TrendMD:

CHENG Gui-Fang, ZHANG Dong-Mei, DING Min, QU Hai-Yun, HE Pin-Gang, FANG Yu-Zhi . Studies on the Interaction of Daunomycin and the Specific Sequence of DNA[J]. Chem. J. Chinese Universities, 2003, 24(8): 1395.

References

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