Studies on Quantum Dots Synthesized in Aqueous Solution for Biological Labeling

Chem. J. Chinese Universities ›› 2003, Vol. 24 ›› Issue (2): 216.

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Studies on Quantum Dots Synthesized in Aqueous Solution for Biological Labeling

LIN Zhang-Bi¹, SU Xing-Guang¹, ZHANG Hao², MU Ying³, SUN Ye³, HU Hai¹, YANG Bai², YAN Gang-Lin³, LUO Gui-Min³, JIN Qin-Han¹

1. Institute of Analytical Science, College of Chemistry, Changchun R & D Center for Analytical Instruments;
2. Key Lab for Supramolecular Structure and Materials;
3. Key Lab for Molecular Enzymology and Engineering of Ministry of Education, Jilin University, Changchun 130023, China

Received:2002-07-02 Online:2003-02-24 Published:2003-02-24

Abstract

Abstract: CdTe semiconductor nanoparticles were synthesized in aqueous solution by using mercaptopropyl acid(HS-CH₂CH₂COOH) as the stabilizer. The nanocrystals exhibit a strong, stable and narrow band luminescence(FWHM=40 nm). The average size estimated from TEM graph is ca. 3 nm. The applicability of the resulting mercaptopropyl acid modified CdTe nanoparticles to the labeling of biomoleculestrypsin was studied in this paper. The absorption spectrum of CdTe-trypsin solution was flatter than that of CdTe nanoparticles over the wavelength range of 400 to 600 nm. While the emission spectrum of CdTe-trypsin solution shows a blue-shift in the emission peak, the intrinsic emission band width is unchanged. It was proved that the shift of the emission peak was the result of the conjugation between CdTe nanoparticles and trypsin, but not of the oxidation caused by O₂. Furthermore, heating could accelerate the speed of conjugation between CdTe nanoparticles and trypsin.

Key words: Quantum dot, Trypsin, Biological fluorescent labeling

CLC Number:

O657

TrendMD:

LIN Zhang-Bi, SU Xing-Guang, ZHANG Hao, MU Ying, SUN Ye, HU Hai, YANG Bai, YAN Gang-Lin, LUO Gui-Min, JIN Qin-Han . Studies on Quantum Dots Synthesized in Aqueous Solution for Biological Labeling[J]. Chem. J. Chinese Universities, 2003, 24(2): 216.

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Studies on Quantum Dots Synthesized in Aqueous Solution for Biological Labeling

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