A Novel Capacitive Immunosensor For Direct Detection of Laminine with a Potentiostatic Step

Chem. J. Chinese Universities ›› 2003, Vol. 24 ›› Issue (1): 46.

• Preface • Previous Articles Next Articles

A Novel Capacitive Immunosensor For Direct Detection of Laminine with a Potentiostatic Step

JIANG De-Chen¹, TANG Jia¹, LIU Bao-Hong¹, SHEN Xian-Rong³, ZHANG Song¹, YANG Peng-Yuan¹, LI Ai-Zhen², HUANG Yi-Ping², KONG Ji-Lie¹

1. Department of Chemistry, Fudan University, Shanghai 200433, China;
2. Department of Microelectronics, Fudan University, Shanghai 200433, China;
3. Shanghai Naval Medical Research Institute, Shanghai 200433, China

Received:2002-01-06 Online:2003-01-24 Published:2003-01-24

Abstract

Abstract: Laminine(Ln) has been identified as an important marker of liver fibrosis or cirrhosis. The Ln immunosensor was formed with thioglycolic acid to produce a self-assembled monolayer on the gold electrode surface. The monoclonal Ln antibody was orderly immobilized on the modified gold surface covalently using EDC/NHS. An unlabeled antigen combined on the antibody could reduce the capacitance of the electrode, which was evaluated by the potentiostatic method. The measurement was performed in microseconds. Ln antigen could be determined in the range of 2-50 ng/mL. The quick response made the immunosensor a promising clinical screening method.

Key words: Laminine, Immunosensor, Self-assembly, Potentiostatic step

CLC Number:

O657

TrendMD:

JIANG De-Chen, TANG Jia, LIU Bao-Hong, SHEN Xian-Rong, ZHANG Song, YANG Peng-Yuan, LI Ai-Zhen, HUANG Yi-Ping, KONG Ji-Lie. A Novel Capacitive Immunosensor For Direct Detection of Laminine with a Potentiostatic Step[J]. Chem. J. Chinese Universities, 2003, 24(1): 46.

[1]	LI Lin, QI Fenglian, QIU Lili, MENG Zihui. Dynamic Amorphous Photonic Structure Patterns Assembled by Hexagonal Magnetic Nanosheets [J]. Chem. J. Chinese Universities, 2022, 43(8): 20220123.
[2]	WU Yushuai, SHANG Yingxu, JIANG Qiao, DING Baoquan. Research Progress of Controllable Self-assembled DNA Origami Structure as Drug Carrier [J]. Chem. J. Chinese Universities, 2022, 43(8): 20220179.
[3]	YU Bin, CHEN Xiaoyan, ZHAO Yue, CHEN Weichang, XIAO Xinyan, LIU Haiyang. Graphene Oxide-based Cobalt Porphyrin Composites for Electrocatalytic Hydrogen Evolution Reaction [J]. Chem. J. Chinese Universities, 2022, 43(2): 20210549.
[4]	LI Bo, MENG Yuxi, WANG Wenwen, ZANG Hongying. Synthesis and Proton Conductivity of Polynuclear Polyoxothiomolybdate Compound [J]. Chem. J. Chinese Universities, 2022, 43(1): 20210657.
[5]	DU Shunfu, WANG Wenjing, EL⁃SAYED El⁃Sayed M., SU Kongzhao, YUAN Daqiang, HONG Maochun. A Chemiluminescent Zirconocene Coordination Tetrahedron [J]. Chem. J. Chinese Universities, 2022, 43(1): 20210628.
[6]	XUE Jin, CAO Xiaowei, LIU Yifan, WANG Min. Preparation of Paper Hollow Gold Nanocage SERS Sensor and Its Rapid and Highly Sensitive Detection for miRNAs in Sputum of Patients with Non-small Cell Lung Cancer [J]. Chem. J. Chinese Universities, 2021, 42(8): 2393.
[7]	ZHANG Juan, HU Xinyue, WANG Hongbo, LIAN Ying, LE Jinyu, YANG Zihao. Crystal-like Hydrogels Consisting of Parallel Hexahedrons Obtained from the Self-assembly ofβ⁃Cyclodextrin/perfluorononanoic Acid Inclusion Complexes [J]. Chem. J. Chinese Universities, 2021, 42(10): 3187.
[8]	TANG Wentao, LI Shengkai, WANG Shen, CHEN Long, CHEN Zhuo. Laser-mediated Enrichment Based Surface Enhanced Raman Analysis [J]. Chem. J. Chinese Universities, 2021, 42(10): 3054.
[9]	HOU Chunxi, LI Yijia, WANG Tingting, LIU Shengda, YAN Tengfei, LIU Junqiu. Application of Elastin-like Polypeptides in Supramolecular Assembly ^† [J]. Chem. J. Chinese Universities, 2020, 41(6): 1163.
[10]	BAI Ruonan, LI Qing, QIAO Shanlin, ZHANG Chunhuan, ZHAO Yongsheng. Controlled Preparation and Optical Waveguide Property of 1,4-Dicarbazolidinylbenzene Microwires ^† [J]. Chem. J. Chinese Universities, 2020, 41(5): 967.
[11]	WANG Jun, WANG Tie. Recent Progress in Functional Nanomaterials Based on Self-assembly Technology ^† [J]. Chem. J. Chinese Universities, 2020, 41(3): 377.
[12]	LI Dong,SUN Yinghui,WANG Zhongshun,HUANG Jing,Lü Nan,JIANG Lin. Large-scale Multiplexed Surface Plasmonic Gold Nanostructures Based on Nanoimprint and Self-assembly ^† [J]. Chem. J. Chinese Universities, 2020, 41(2): 221.
[13]	GAO Miaomiao,WANG Chenglong,DOU Hongjing,XU Guoxiong. One-step Self-assembly/polymerization Fabrication and Biomedical Application of Carboplatin@Dextran Nanocarrier^† [J]. Chem. J. Chinese Universities, 2019, 40(6): 1301.
[14]	WANG Yanhui,ZOU Qingzhi,ZHU Youliang,FU Cuiliu,HUANG Yineng,LI Zhanwei,SUN Zhaoyan. Simulation Study on the Self-assembly of Softtriblock Janus Particles^† [J]. Chem. J. Chinese Universities, 2019, 40(5): 1037.
[15]	WANG Wen,TAO Xiafang,WU Yunyan,ZHAO Nan,CHENG Xiaonong,YANG Juan,ZHOU Yazhou. Fabrication and SERS Performance of “Sandwich” Structured Silver Nanoparticles/Graphene Oxide Substrates^† [J]. Chem. J. Chinese Universities, 2019, 40(4): 667.

A Novel Capacitive Immunosensor For Direct Detection of Laminine with a Potentiostatic Step

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments