Studies on the Interaction of Porphyrin with Antibody with Fluorescent Spectrum and UV-Vis Differential Spectra

Abstract

Abstract: After meso tetra (α, α, α, α-O-phenylacetyl benzene)porphyrin combined with McAb1F2, there was a significant hyperchromic effect, indicating that the combination of porphyrin and antibody is rigid and compact, aromatic amino acids exist at the combining sites of antigen in antibody. These aromatic amino acids are Trys and Trps, but the numbers of Trp are more than that found for Trys. The stochiometric ratio of porphyrin to 1F2 is 1:1, the disassociation constant was determined as (2.084 ±0.246)×10^-10 mol/Lby a method of fluorescence quenching, showing that both have a high affinity.

Key words: Porphyrin, Antibody, UV-Vis differential spectra, Fluorescent spectra

CLC Number:

O657.3

TrendMD:

QI Chao, LI Wei-Guo, ZHANG Yu-Jing, LIU Li-Yan, ZHAO Da-Qing. Studies on the Interaction of Porphyrin with Antibody with Fluorescent Spectrum and UV-Vis Differential Spectra[J]. Chem. J. Chinese Universities, 2002, 23(8): 1453.

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Studies on the Interaction of Porphyrin with Antibody with Fluorescent Spectrum and UV-Vis Differential Spectra

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