Sensor Array Based on MTPP-AuNPs Compounds for Fast Identification of Organic Small Molecules in Exhaled Breath of Lung Cancer Patients†

doi:10.7503/cjcu20140648

Abstract

Abstract:

The smaller gold nanoparticles(AuNPs) were synthesized and used as a protectant for metal tetraphenylporphyrins(MTPP), and a 6×6 sensor array was constructed together by AuNPs-MTPP compounds and pH indicators to detect 12 organic small molecules(OSMs) in exhaled breath of lung cancer patients with three concentration gradients, and each test was repeated five times. The protective effect showed that the stability and sensitivity of the array were significantly enhanced due to the introduction of AuNPs; hierarchical cluster analysis(HCA) indicated that the results of five parallel experiments gathered into a cluster firstly, and target objects with similar structure were gotten together subsequently; digital color difference maps of OSMs with different kinds and concentrations visually displayed that the proposed method had good capability for qualitative and semi-quantitative analysis. The method can be used for fast identification of OSMs and has potential application prospect in lung cancer screening.

Key words: Array sensor, Gold nanoparticles-metal tetraphenylporphyrins(AuNPs-MTPP), Rapid identification, Organic small molecules(OSMs) in exhaled breath, Lung cancer

CLC Number:

O657

TrendMD:

XU Yonghong, HUO Danqun, FA Huanbao, YANG Mei, HOU Changjun. Sensor Array Based on MTPP-AuNPs Compounds for Fast Identification of Organic Small Molecules in Exhaled Breath of Lung Cancer Patients^†[J]. Chem. J. Chinese Universities, 2015, 36(2): 241.

Figures/Tables 7

Fig.1 Gas flow diagrams of detection system(A), structure schematic diagram of chamber(B) and response mechanism(C) of sensor’s response to gas mixture

Table 1 Temperatures, saturated vapor pressures, volumes and RSD of 2.16 mg/m3 tested objects

Category	Compound	Temperature/℃	Pressure/Pa	Volume/mL	RSD(%)
Hydrocarbon	Pentane	20	57300	0.04	3.1
	Heptane	20	4800	0.52	2.8
	Undecane	20	140	17.86	3.2
	Isoprene	15.4	53320	0.05	3.0
Alcohols	Methanol	20	12800	0.20	3.3
	Butanol	20	700	3.62	3.6
Aldoketones	Hexanal	20	1150	2.20	3.7
	Heptanal	25	400	6.33	4.9
	Acetone	20	23300	0.11	3.4
Benzenes	Benzene	27	13820	0.18	3.3
	Toluene	27	4180	0.61	4.5
	Styrene	20	600	4.22	5.0

Fig.2 UV-Vis absorbance spectra(A), digital photos(B) of AuNPs(a, a) and HAuCl4·4H2O(b, b), fluorescence spectrum(C) and SEM image(D) of AuNPs in aqueous solution

Fig.3 Protective effects of AuNPs, array(for 2.16 mg/m3 benzene) stored under N2 during different lay-out time Digital color difference maps with/without protection of AuNPs(A) and the Euclidean distance with(B)/without(C) protection of AuNPs.

Scheme 1 Interaction between MTPP and AuNPs via non-bonding electrons on nitrogens of MTPP

Fig.4 Digital color difference maps of AuNPs-MTPP sensor array for 12 OSMs(2.16 mg/m3)

Fig.5 Hierarchical cluster analysis for 12 OSMs with 2.16 mg/m3 Insets are difference maps of hexanal at three concentrations.

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