Two-photon Fluorescent Labeling Kit for the Real-time Dynamic Biopsy of CA19-9†

doi:10.7503/cjcu20150727

Chem. J. Chinese Universities ›› 2016, Vol. 37 ›› Issue (4): 638.doi: 10.7503/cjcu20150727

• Analytical Chemistry • Previous Articles Next Articles

Two-photon Fluorescent Labeling Kit for the Real-time Dynamic Biopsy of CA19-9^†

HUANG Chibao^1,^2,^*(), ZHANG Daohai³, ZENG Boping¹, LIU Qibin³, CHEN Huashi¹, KANG Shuai¹, CHEN Xiaoyuan²

1. Chemistry and Chemical Engineering College, Zunyi Normal University, Zunyi 563002, China
2. College of Agricultural Science and Engineering, Shaoguan University, Shaoguan 512005, China
3. College of Materials and Metallurgy, Guizhou University, Guiyang 550025, China

Received:2015-09-17 Online:2016-04-10 Published:2016-03-10
Contact: HUANG Chibao E-mail:huangchibao@163.com
Supported by:
† Supported by the National Natural Science Foundation of China(Nos:21562050, 11464052), the Special Fund Project of the Construction of the Eighth Batch of Scientific and Technological Innovation Talent Team in Guizhou Province, China[No:(2015)4007], Guizhou Provincial Science and Technology Fund Project, China(Nos:J[2015]2146, J[2012]2345), the Key Project of Education Department of Guizhou Province(Nos:KY[2014]296), KY(2013)171), the Teaching Contents and Curriculum System Reform Project of Higher Education in Guizhou Province, China(No.KY [2014] JXGChcb), the Project of “15851 Talents Elite Project” in Zunyi City, China[No:(2015)4007] and the Science and Technology Project of Zunyi City Honghuagang District, China(No.[2015]18)

Abstract

Abstract:

Carbohydrate antigen CA19-9(Ag) is a tumor marker associated with pancreatic cancer, gallbladder cancer, colon cancer and gastric cancer in early stage of tumor and cancer stage. Antibody Ab19-9(Ab) to carbohydrate antigens were labeled to form probe-labeled antibody LP-Ab using the prepared two-photon fluorescence probe LP. On the basis of the antigen-antibody specificity affinity principle, LP-Ab could combine with carbohydrate antigen Ag into a complex LP-Ab·Ag, which was excited by near infrared laser(740 nm) and emitted strong two-photon fluorescence to accomplish the high sensitive biopsy and real-time dynamic high-resolution imaging of the tumor marker Ag with two-photon fluorescence microscope. Based on this, a simple and convenient two-photon fluorescent labeling kit was prepared to realize the rapid detection of tumor, which is convenient for large-scale clinic testing and screening. The research further broadens the application of LP, and provides a sharp tool for revealing the mechanism of cancer occurrence, development and metastasis. LP-Ab can be used to observe and study the dynamic transfer mechanisms of the tumor markers in cells and the mechanism of carcinogenesis.

Key words: Carbonhydrate antigen CA19-9, Antibody Ab19-9, Immunofluorescence labeling, Two-photon, Biopsy(Ed.: P, H, N, K)

CLC Number:

O657

TrendMD:

HUANG Chibao, ZHANG Daohai, ZENG Boping, LIU Qibin, CHEN Huashi, KANG Shuai, CHEN Xiaoyuan. Two-photon Fluorescent Labeling Kit for the Real-time Dynamic Biopsy of CA19-9^†[J]. Chem. J. Chinese Universities, 2016, 37(4): 638.

Figures/Tables 5

Fig.1 Molecular structure of LP

Fig.2 Two-photon action spectra of LP(a, 10 μmol/L) and LP-Ab(b, 10 μmol/L)

Fig.3 TPM images of 1 μmol/L LP-Ab-labeled mouse pancreatic carcinoma cells collected at 450—500 nm^(A) Magnification at 10×; (B) magnification at 40×. The images were collected upon excitation at 740 nm with a femtosecond pulse. Cells shown are representative images from replicate experiments(n=5).

Fig.4 Negative(A) and positive(B) fluorescent images of 1 μmol/L LP-Ab-labeled mouse pancreatic carcinoma and normal cells under the eyepiece

Fig.5 Diagram of two-photon fluorescent labeling kit

References 30

[1]	Gu Y. L., Wang X. C., Jin C. G., Chen Y., Wu Z. P., Huang Y. K., Cancer Research and Clinic, 2005, 17(5), 329—331
	(谷玉兰, 王熙才, 金从国, 陈艳, 伍治平, 黄焱坤. 肿瘤研究与临床, 2005, 17(5), 329—331)
[2]	Katz A., Hanlon A., Lanciano R., Int. J. Radiat. Oncol. Biol. Phys., 1998, 41, 393—396
[3]	Steinberg W., Am. J. Gastroenterol., 1990, 85(4), 350—355
[4]	Yoshikawa T., Nishida K., Tanigawa M., Digestion, 1985, 31(2/3), 67—76
[5]	Koprowski H., Steplewski Z., Mitchell K., Somatic Cell Genet, 1979, 5(6), 957—971
[6]	Lin J., Yan F., Hu X., Ju H., J. Immunol. Methods, 2004, 291(1/2), 165—174
[7]	Yang S.M., Cong H., Wang J. X., Wang H. M.,Journal of Modern Laboratory Medicine, 2013, (2), 126—128
	(杨曙梅, 丛辉, 王建新, 王惠民. 现代检验医学杂志, 2013, (2), 126—128)
[8]	Zhai S. D., Zhao R. S., Yan B. X., Liu H. Y., Chinese Journal of New Drugs, 2003, 12(3), 194—196
	(翟所迪, 赵荣生, 严宝霞, 刘红鹰. 中国新药杂志, 2003, 12(3), 194—196)
[9]	Yu X. W., Wang Y. N., Cheng B. J., Ma L. D., Laboratory Medicine, 2013, 28(1), 76—79
	(俞小卫, 王亚楠, 程宝金, 马玲娣. 检验医学, 2013, 28(1), 76—79)
[10]	Liang F. H., Wang D., Ma P. Y., Wang X. H., Song D. Q., Yu Y., Chem. Res. Chinese Universities, 2015, 31(5), 724—729
[11]	Guan J., Zhang P., Wei T., Lin Q., Yao H., Zhang Y. M., Chem. Res. Chinese Universities, 2015, 31(3), 347—351
[12]	Ojida A., Mito-oka Y., Inoue M., J. Am. Chem. Soc., 2002, 124(22), 6256—6258
[13]	Minta A., Kao J. P. Y., Tsien R. Y., J. Biol. Chem., 1989, 264(14), 8171—8178
[14]	Werts M. H. W., Gmouh S., Mongin O., J. Am. Chem. Soc., 2004, 126(50), 16294—16295
[15]	Kim H. M., Jeong M. Y., Ahn H. C., J. Org. Chem., 2004, 69(17), 5749—5751
[16]	Huang C. B., Liang X., Zeng Q. H., Chen H. S., Zeng B. P., Yi D. S., Chen X. Y., Chem. J. Chinese Universities, 2015, 36(4), 646—653
	(黄池宝, 梁兴, 曾启华, 陈华仕, 曾伯平, 易道生, 陈晓远. 高等学校化学学报, 2015, 36(4), 646—653)
[17]	Zhou S. S., Xue X., Wei D., Jiang B., Wang J. F., Lu C. H., Chem. Res. Chinese Universities, 2012, 28(4), 647—651
[18]	Lei H., Huang Z. L., Wang H .Z., Physics, 2003, 32(1), 19—26
	(雷虹, 黄振立, 汪河洲. 物理, 2003, 32(1), 19—26)
[19]	Belfield K. D., Bondar M. V., Przhonska O. V., Schafer K. J., J. Photochem. Photobiol. A: Chem., 2004, 162, 489—496
[20]	Belfield K. D., Bondar M. V., Przhonska O. V., Schafer K. J., J. Photochem. Photobiol. A: Chem., 2004, 162, 569—574
[21]	Belfield K. D., Schafer K. J., Mourad W., Reinhardt B. A., J. Org. Chem., 2000, 65, 4475—4481
[22]	Belfield K. D., Morales A. R., Hales J. M., Hagan D. J., Van Stryland E. W., Chapela V. M., Percino J., Chem. Mater., 2004, 16, 2267—2273
[23]	Belfield K. D., Morales A. R., Kang B. S., Hales J. M., Hagan D. J., Van Stryland E. W., Chapela V. M., Percino J., Chem. Mater., 2004, 16, 4634—4641
[24]	Morales A. R., Belfield K. D., Hales J. M., Hagan D. J., van Stryland E. W., Chem. Mater., 2006, 18, 4972—4980
[25]	Yao S., Belfield K. D., J. Org. Chem., 2005, 70, 5126—5132
[26]	Morales A.R., Yanez C. O., Schafer-Hales K. J., Marcus A. I., Belfield K. D., Bioconjugate Chem., 2009, 20, 1992—2000
[27]	Albota M., Beljonne D., Bredas J. L., Ehrlich J. E., Fu J. Y., Heikal A. A., Hess S. E., Kogej T., Levin M. D., Marder S. R., McCord-Maughon D., Perry J. W., Rockel H., Rumi M., Subramaniam G., Webb W. W., Wu X. L., Xu C., Science, 1998, 281(5383), 1653—1656
[28]	Day P. N., Nguyen K. A., Pachter R., J. Phys. Chem. B, 2005, 109(5), 1803—1814
[29]	Pond S. J. K., Tsutsumi O., Rumi M., Kwon O., Zojer E., Jean-Luc B., Marder S. R., Perry J. W., J. Am. Chem. Soc., 2004, 126(30), 9291—9306
[30]	Xu C., Webb W. W., J. Opt. Soc. Am. B, 1996, 13(3), 481—491

Two-photon Fluorescent Labeling Kit for the Real-time Dynamic Biopsy of CA19-9^†

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