Immobilization of Plant Protoplast on Gold Electrode Surface

Chem. J. Chinese Universities ›› 2002, Vol. 23 ›› Issue (7): 1392.

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Immobilization of Plant Protoplast on Gold Electrode Surface

ZHANG Hong-Ping¹, QI Yu-Lan¹, YAN Man-Ming¹, WEI Yi², LIN Zhong-Hua³, JIANG Zhi-Yu¹

1. Department of Chemistry;
2. Academy of Life Science, Fudan University, Shanghai 200433, China;
3. Department of Chemistry, State Key Laboratory for Physical Chemistry of the Solid Surface, Xiamen University, Xiamen 361005, ChinaLaborato

Received:2001-08-20 Online:2002-07-24 Published:2002-07-24

Abstract

Abstract: Barley protoplast was immobilized on poly(dially-dimethylammonium) (PDADMAC) modified gold electrode via the electrostatic attraction between the positively charged amine groups of PDADMACand the negatively charged barley protoplast. STMimage reveals that PDADMAC/Au(111) surface displays a nano-organized dot(4-6 nm) array structure. The processes of the PDADMACadsorption and of the barley protoplast immobilization were monitored from the in situ QCMfrequency change. The effects of the adsorbed mass and of the density and viscosity of medium on the frequency change of QCMwere discussed, respectively. The QCM result shows that the thickness of the adsorbed PDADMAClayer is 2.4 nm.

Key words: Polyelectrolyte, Barley protoplast, Immobilization

CLC Number:

O646

TrendMD:

ZHANG Hong-Ping, QI Yu-Lan, YAN Man-Ming, WEI Yi, LIN Zhong-Hua, JIANG Zhi-Yu . Immobilization of Plant Protoplast on Gold Electrode Surface[J]. Chem. J. Chinese Universities, 2002, 23(7): 1392.

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Immobilization of Plant Protoplast on Gold Electrode Surface

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