pH-Dependent Specific Protein Recognition Ability of Glycopolymers†

doi:10.7503/cjcu20150350

Abstract

Abstract:

A new di-block glycopolymer, poly(gluconamidoethylmethacrylate)-b-poly[2-(diethylamino)ethyl methacrylate]-co-poly[2-(dimethylamino)ethyl methacrylate][PGAMA-b-P(DMAEMA-co-DEAEMA), PGME] was synthesized. The chemical composition and the molecular weight of the glycopolymer were characterized by nuclear magnetic resonance(¹H NMR) and gel permeation chromatography(GPC). The interactions of PGME and proteins were investigated by monitoring the turbidity value of the mixture solutions. PGME showed the specific biomolecular recognition ability with Concanavalin A compared with bovine serum albumin(BSA) at pH=8.3. When the environmental pH value changed to 7.3, the ability of PGME specific biomolecular recognition was suppressed and PGME exhibited the behavior of non-specific binding with bovine serum albumin and Concanavalin A. Dynamic light scattering and atomic force microscopy(AFM) tests were performed to investigate the properties of the PGME-protein complex aggregations, it would be a promising to design pH-sensitive drug delivery system of saccharides.

Key words: Glycopolymer, pH-responsibility, Protein, Polyelectrolyte

CLC Number:

O632

TrendMD:

SONG Rongguang, ZHOU Yihan, LI Fan, WANG Haiyan, SHI Tongfei, ZHANG Guo. pH-Dependent Specific Protein Recognition Ability of Glycopolymers^†[J]. Chem. J. Chinese Universities, 2015, 36(12): 2604.

Figures/Tables 11

Scheme 1 Synthesis of PGME

Fig.1 1H NMR spectrum of PGME

Fig.2 GPC trace of PGME

Fig.3 Interactions of BSA and Con A with PGME at pH=8.3 in 0.02 mol/L PBS

Fig.4 Interactions of BSA and Con A with PGME at pH=7.3 in 0.02 mol/L PBS

Fig.5 Interactions of BSA with PGME in 0.02 mol/L PBS with various pH

Fig.6 Interactions of BSA with PGME while pH cycled in 7.0 and 8.0 in 0.02 mol/L PBS

Fig.7 Typical hydrodynamic diameter distributions[f(Rh)], obtained at 25 ℃ for aqueous solution of Con A and PGME with pH=8.3 BSA; Con A; BSA-glycopolymer; Con A-glycopolymer.

Fig.8 Typical hydrodynamic diameter distributions[f(Rh)], obtained at 25 ℃ for aqueous solution of Con A and PGME with pH=7.3 BSA; Con A; BSA-glycopolymer; Con A-glycopolymer.

Fig.9 AFM images of PGME-BSA aggregations at pH=7.3(A) and PGME-Con A aggregations at pH=8.3(B)

Fig.10 Fluorescence emission spectra of Con A in the presence of different concentrations of PGME at pH=8.0 Concentration of glycopolymer(mg/mL): 0.01; 0.02; 0.05; 0.1; 0.5.

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